PCR-Dipstick-Oriented Surveillance and Characterization of mcr-1 - and Carbapenemase-Carrying Enterobacteriaceae in a Thai Hospital

Frontiers in Microbiology
Rathina Kumar ShanmugakaniShigeyuki Hamada

Abstract

Colistin is used as an alternative therapeutic for carbapenemase-producing Enterobacteriaceae (CPE) infections which are spreading at a very high rate due to the transfer of carbapenemase genes through mobile genetic elements. Due to the emergence of mcr-1, the plasmid-mediated colistin resistance gene, mcr-1-positive Enterobacteriaceae (MCRPEn) pose a high risk for the transfer of mcr-1-carrying plasmid to CPE, leading to a situation with no treatment alternatives for infections caused by Enterobacteriaceae possessing both mcr-1 and carbapenemase genes. Here, we report the application of PCR-dipstick-oriented surveillance strategy to control MCRPEn and CPE by conducting the PCR-dipstick technique for the detection of MCRPEn and CPE in a tertiary care hospital in Thailand and comparing its efficacy with conventional surveillance method. Our surveillance results showed a high MCRPEn (5.9%) and CPE (8.7%) carriage rate among the 219 rectal swab specimens examined. Three different CPE clones were determined by pulsed-field gel electrophoresis (PFGE) whereas only two MCRPEn isolates were found to be closely related as shown by single nucleotide polymorphism-based phylogenetic analysis. Whole genome sequencing (WGS) and plasmid anal...Continue Reading

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Citations

Jul 10, 2020·Lab on a Chip·Rathina Kumar ShanmugakaniSaurabh Mehta
Apr 4, 2021·Pathogens·Nori YoshiokaKazunori Tomono

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Datasets Mentioned

BETA
DRX131210
DRX131211
DRX131212
DRX131213
DRX131214
DRX131215
DRX131216
DRX131217
DRX131218
DRX131219

Methods Mentioned

BETA
PCR
electrophoresis

Software Mentioned

GraphPad
CSI Phylogeny
GraphPad Prism
MLST
StataMP
CLC Genomics Workbench
BLAST
PlasmidFinder
ResFinder

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