PCR fingerprinting of Blastocystis isolated from symptomatic and asymptomatic human hosts.

Parasitology Research
T C TanH V Smith

Abstract

Genomic DNA from 16 Blastocystis hominis isolates comprising of eight asymptomatic isolates (A1-A8) and eight symptomatic isolates (S1-S8) was amplified by arbitrarily primed polymerase chain reaction (AP-PCR) using 38 arbitrary 10-mer primers. Six primers (A10, B5, C20, D1, F6, and F10) generated reproducible DNA fingerprints. AP-PCR amplification revealed similar DNA fingerprints among all symptomatic isolates (S1-S8) with common bands at 850 bp using primer A10, 920 bp using primer B5, and 1.3 kbp using primer D1. Isolates A1, A3, A4, A5, A6, and A7 showed similar DNA banding patterns and all asymptomatic isolates (A1-A8) shared a major band at 1 kbp using primer B5. Isolates A2 and A8 showed distinct DNA banding patterns that differed from the remainder of the isolates. The results of the phylogenetic analyses showed that all symptomatic isolates (S1-S8) formed a clade with >70% similarity among the isolates and which were clearly separate from asymptomatic isolates A1, A3, A4, A5, A6, and A7. Asymptomatic isolates A2 and A8 formed two distinct and separate clades. AP-PCR revealed higher genetic variability within the asymptomatic isolates than within the symptomatic isolates. The present study suggests that AP-PCR can be a...Continue Reading

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Citations

Aug 1, 2007·Parasitology Research·Yiming YanXiaobing Luo
Oct 22, 2008·Parasites & Vectors·Kenneth F BooromMorris S Jones
Jan 23, 2010·American Journal of Clinical Pathology·Constantine M VassalosNicholas Vakalis
Nov 18, 2008·Trends in Parasitology·C Rune StensvoldHuw V Smith
Jan 24, 2007·Trends in Parasitology·C Rune StensvoldC Graham Clark
May 23, 2015·Parasitology Research·Robyn NagelRebecca J Traub

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