PMID: 9546158Apr 18, 1998Paper

PCR for detection of Shigella spp. in mayonnaise

Applied and Environmental Microbiology
E Villalobo, A Torres

Abstract

The use of PCR to amplify a specific virA gene fragment serves as a highly specific and sensitive method to detect virulent bacteria of the genus Shigella and enteroinvasive Escherichia coli. Amplification of a 215-bp DNA band was obtained by using isolated genomic DNA of Shigella, individual cells of Shigella dysenteriae, and mayonnaise contaminated with S. dysenteriae. Moreover, a multiplex PCR with specific (virA) and bacterium-restricted (16S ribosomal DNA) primers generated an amplification product of approximately 755 bp for all bacteria tested and an additional 215-bp product for Shigella and enteroinvasive E. coli.

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Citations

Jan 27, 2006·World Journal of Gastroenterology : WJG·Lian-Qun JinZheng-Quan Yuan
Dec 18, 2009·International Journal of Microbiology·Hiroshi FukushimaYuko Akiba
Mar 20, 2013·BioMed Research International·Suvash Chandra OjhaKirnpal-Kaur Banga Singh
Sep 6, 2000·Letters in Applied Microbiology·A VantarakisM Papapetropoulou
Jul 5, 2012·Revista Da Sociedade Brasileira De Medicina Tropical·Hectorina RodulfoMiriam Michelli
Jan 15, 2020·PloS One·Aviaja L HauptmannDennis S Nielsen
Jul 28, 2020·Brazilian Journal of Microbiology : [publication of the Brazilian Society for Microbiology]·Júlia Cunha GonzalesJuliana Pfrimer Falcão
May 12, 2007·Applied Microbiology and Biotechnology·Xin-Wei WangJun-Wen Li
Sep 7, 2006·Critical Reviews in Food Science and Nutrition·B R WarrenK R Schneider
Feb 24, 2016·Japanese Journal of Infectious Diseases·Cecilia CasabonneClaudia Balagué
Feb 6, 2010·Journal of Food Protection·Wen S LinKhanh T Van
Nov 10, 2017·Journal of Clinical Microbiology·Jie LiuEric Houpt
Jul 26, 2018·Journal of Food Science and Technology·Bianca Mendes MacielGeorge Rêgo Albuquerque
Mar 8, 2005·Journal of Clinical Microbiology·Melissa M NicolasMark D Schrenzel
Nov 8, 2003·Journal of Clinical Microbiology·Hiroshi FukushimaRyotaro Seki

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