PCR for the Specific Detection of an Escherichia coli O157:H7 Laboratory Control Strain

Journal of Food Protection
Michael KnowlesBurton W Blais

Abstract

Control strains of bacterial pathogens such as Escherichia coli O157:H7 are commonly processed in parallel with test samples in food microbiology laboratories as a quality control measure to assure the satisfactory performance of materials used in the analytical procedure. Before positive findings can be reported for risk management purposes, analysts must have a means of verifying that pathogenic bacteria (e.g., E. coli O157:H7) recovered from test samples are not due to inadvertent contamination with the control strain routinely handled in the laboratory environment. Here, we report on the application of an in-house bioinformatic pipeline for the identification of unique genomic signature sequences in the development of specific oligonucleotide primers enabling the identification of a common positive control strain, E. coli O157:H7 (ATCC 35150), using a simple PCR procedure.

References

Oct 5, 1990·Journal of Molecular Biology·S F AltschulD J Lipman
Dec 1, 2010·Genome Biology·David R KelleySteven L Salzberg
Apr 18, 2012·Journal of Computational Biology : a Journal of Computational Molecular Cell Biology·Anton BankevichPavel A Pevzner
Nov 22, 2013·Nucleic Acids Research·UNKNOWN NCBI Resource Coordinators
Jan 30, 2015·International Journal of Antimicrobial Agents·Mohamed NawazRoger Steele

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Citations

Feb 22, 2017·Journal of Food Protection·Michael KnowlesBurton Blais
Mar 22, 2019·Frontiers in Microbiology·Emily E F BrownBurton Blais

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