PCR of crtNM combined with analytical biochemistry: An efficient way to identify carotenoid producing lactic acid bacteria

Systematic and Applied Microbiology
Williams TurpinChristèle Humblot

Abstract

Lactic acid bacteria (LAB) synthesize a wide variety of biochemical compounds during food fermentation. Carotenoids provide important biological functions for bacteria, and their consumption by humans has many beneficial effects. In this study, the presence of several genes involved in the production of carotenoids was determined by BLAST analysis and PCR in a collection of 156 LAB isolated from traditional amylaceous African fermented foods. Only the crtE gene and the crtNM operon were present and detected in Lactobacillaceae. Most of the strains with positive PCR detection of the operon crtNM produced carotenoid-like compounds when grown in MRS broth. The carotenoids produced differed from compounds previously identified in other LAB except for one peak, which was closely related to 4,4'-diaponeurosporene already reported in the literature in Lactobacillus plantarum species. Most producing strains belonged to Lactobacillus fermentum and L. plantarum species but a few Pediococcus acidilactici were also producers. Furthermore, the most efficient L. plantarum was able to synthesize carotenoids in a cereal fermented food. Genetic screening was shown to be efficient since, in all cases, it eliminated the need for biochemical analy...Continue Reading

References

Feb 5, 2003·Proceedings of the National Academy of Sciences of the United States of America·Michiel KleerebezemRoland J Siezen
Mar 10, 2005·Microbiology and Molecular Biology Reviews : MMBR·Daisuke UmenoFrances H Arnold
Jul 16, 2005·The Journal of Biological Chemistry·Alexandra PelzFriedrich Götz
Jul 18, 2006·International Journal of Food Microbiology·Nabil Ben OmarAntonio Gálvez
Oct 13, 2006·Proceedings of the National Academy of Sciences of the United States of America·K MakarovaD Mills
May 20, 2008·DNA Research : an International Journal for Rapid Publication of Reports on Genes and Genomes·Hidetoshi MoritaMasahira Hattori
Aug 4, 2009·Journal of Food Science·Sylvie AvallonePhilippe Bohuon
Mar 23, 2010·International Journal of Food Microbiology·Juan Garrido-FernándezJosé Luis Ruiz-Barba
Sep 22, 2011·Journal of Applied Microbiology·J G LeBlancF Sesma
Nov 22, 2011·Molecular Nutrition & Food Research·Yoav SharoniJoseph Levy
Dec 14, 2011·Journal of Bacteriology·Roland J SiezenSacha A F T van Hijum
Jun 8, 2012·PloS One·Williams TurpinJean-Pierre Guyot
Sep 12, 2012·BMC Microbiology·Sabine A E HeiderVolker F Wendisch

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