PCR-restriction fragment length polymorphism analysis of a diagnostic 452-base-pair DNA fragment discriminates between Cryptosporidium parvum and C. meleagridis and between C. parvum isolates of human and animal origin.

Applied and Environmental Microbiology
K GuyotE Dei-Cas

Abstract

Genomic DNAs from human Cryptosporidium isolates previously typed by analysis of the 18S ribosomal DNA locus (Cryptosporidium parvum bovine genotype, C. parvum human genotype, Cryptosporidium meleagridis, and Cryptosporidium felis) were used to amplify the diagnostic fragment described by Laxer et al. (M. A. Laxer, B. K. Timblin, and R. J. Patel, Am. J. Trop. Med. Hyg., 45:688-694, 1991). The obtained 452-bp amplified fragments were sequenced and aligned with the homologous Cryptosporidium wrairi sequence. Polymorphism was exploited to develop a restriction fragment length polymorphism method able to discriminate Cryptosporidium species and C. parvum genotypes.

References

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Citations

Mar 26, 2004·Veterinary Parasitology·Makoto MatsubayashiEiichiroh Baba
Dec 5, 2006·Applied and Environmental Microbiology·Ahmad Reza MeamarMostafa Rezaian
Jan 17, 2004·Clinical Microbiology Reviews·Lihua XiaoSteve J Upton
Apr 20, 2006·Veterinary Parasitology·Ronald FayerJ P Dubey

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