PCR-reverse blot hybridization assay for fast and accurate identification of causative species in superficial fungal infections

Clinical and Experimental Dermatology
S D ParkE H Choi

Abstract

Superficial fungal infections are a very common problem in dermatological clinics. The diagnostic method of fungal culture is time-consuming and has inconsistent sensitivity. Therefore, a practical method for rapid and accurate identification of the species causing superficial fungal infections is needed. To compare PCR-reverse blot hybridization assay (PCR-REBA) with conventional fungal diagnostic methods so as to determine the reliability of PCR-REBA for the diagnosis and species identification in superficial fungal infections. Potassium hydroxide (KOH) preparation, fungal culture, conventional real-time PCR and PCR-REBA were used to assess 83 specimens, and the results from each method were compared. Of the 83 specimens, 44 specimens that were positive by fungal culture had 62.7% agreement with PCR-REBA. Compared with real-time PCR, there was 68.7% agreement with fungal culture, but 91.6% agreement with PCR-REBA. When the comparison was made using the 55 specimens that gave positive results in both KOH preparation and fungal culture, there was 85.5% agreement with real-time PCR for fungal culture, but 94.5% agreement with PCR-REBA. Compared with KOH preparation or fungal culture, PCR-REBA has higher sensitivity and specifici...Continue Reading

References

Aug 2, 2003·Journal of the American Academy of Dermatology·Jeffrey M WeinbergLily Najarian
Jun 27, 2006·Dermatologic Clinics·Gérald E PiérardJorge E Arrese
May 15, 2008·Mycopathologia·Raymond Robert, Marc Pihet
Mar 5, 2010·Journal of Clinical Microbiology·Christiane HonischStefan Niemann
Mar 30, 2010·Clinics in Dermatology·Oliverio WelshEsperanza Welsh
Mar 30, 2010·Clinics in Dermatology·Mahreen Ameen
Feb 18, 2011·Journal of Clinical Microbiology·Anke PierikAdriaan J C van den Brule
Jan 18, 2012·Current Opinion in Infectious Diseases·Rasmus H Jensen, Maiken C Arendrup
Jan 24, 2013·Journal of Korean Medical Science·Won-Jeong KimMoon-Bum Kim
Feb 2, 2013·Mycopathologia·Iman HaghaniSeyed Reza Aghili
Feb 13, 2013·Journal of Cutaneous Pathology·Munir H IdrissDirk Elston
Mar 1, 2013·Journal of Clinical Microbiology·Yeonim ChoiHyeyoung Lee
Oct 2, 2013·Clinics in Dermatology·Aditya K Gupta, Fiona C Simpson

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.