PMID: 9446909Feb 3, 1998Paper

PCR-RFLP pattern of three kinds of Metagonimus in Korea

The Korean Journal of Parasitology
J R YuJ Y Chai

Abstract

We tried to compare the three kinds of Metagonimus species, M. yokogawai, Metagonimus Miyata type, and M. takahashii, which were known to be distributed in Korea with polymerase chain reaction based-restriction fragment length polymorphism (PCR-RFLP) patterns. We amplified the internal transcribed spacer 1 (ITS1) site of ribosomal RNA and mitochondrial cytochrome c oxidase I (mCOI) gene. The restriction patterns of ITS1 gene with Rsa I, Alu I and Msp I showed multiple fragmented bands of different sizes between three kinds of Metagonimus. In case of mCOI gene, Rsa I and Alu I enzymes produced differentially fragmented band patterns. According to the parsimony analysis of PCR-RFLP patterns, the estimated genetic divergence between M. yokogawai and Metagonimus Miyata type was 0.034880, between Metagonimus Miyata type and M. takahashii was 0.028098, between M. yokogawai and M. takahashii was 0.018179. It is suggested that Metagonimus Miyata type may be separate species and evolutionize at the older time than the other two species.

References

Dec 1, 1990·Kisaengch'unghak chapchi. The Korean journal of parasitology·J Y Chai, S H Lee
Jan 1, 1980·Annual Review of Biochemistry·E O Long, I B Dawid
Dec 1, 1994·The Korean Journal of Parasitology·J R YuS H Lee
Feb 1, 1993·Molecular and Biochemical Parasitology·J Bowles, D P McManus
Jun 1, 1993·The Korean Journal of Parasitology·J Y ChaiS H Lee

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Citations

Jul 13, 2002·Parasitology International·Jong-Yil Chai, Soon-Hyung Lee
Aug 8, 2006·Experimental Parasitology·Pralongyut SripalwitSomboon Anuntalabhochai
Nov 12, 2002·Memórias do Instituto Oswaldo Cruz·Roberta L CaldeiraGuilherme C Oliveira
Apr 1, 2000·The Korean Journal of Parasitology·J Y ChaiS H Lee
Jan 13, 2010·International Journal of Dermatology·Sumiyuki MiiHikaru Eto
Nov 4, 2009·The Korean Journal of Parasitology·Jong-Yil ChaiHan-Jong Rim
Apr 3, 1999·Toxicology and Industrial Health·P Short, T Colborn
Dec 30, 1998·The Korean Journal of Parasitology·J Y ChaiS H Lee
Feb 3, 1998·The Korean Journal of Parasitology·J R YuJ Y Chai
Jul 30, 2014·Asian Pacific Journal of Tropical Medicine·Thapana ChontananarthJong Yil Chai

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