PD-1/PD-L1 regulates Treg differentiation in pregnancy-induced hypertension

Brazilian Journal of Medical and Biological Research = Revista Brasileira De Pesquisas Médicas E Biológicas
Lai JiangLing Chen

Abstract

Pregnancy-induced hypertension (PIH) causes significant maternal and fetal morbidity and mortality. A decreased number of regulatory T (Treg) cells is associated with the pathogenesis of PIH. The programmed cell death-1 (PD-1)/PD-ligand 1 (PD-L1) pathway is critical to normal pregnancy (NP) by promoting Treg cell development. However, the relationship between PD-1/PD-L1 and Treg differentiation in PIH has not been fully elucidated. In this study, venous blood was obtained from 20 NP and 58 PIH patients. Peripheral blood mononuclear cells (PBMCs) were isolated from venous blood. The levels of Treg-related cytokines (TGF-β, IL-10, and IL-35) in serum and PBMCs were measured by ELISA. The percentage of Treg cells in PBMCs was assessed by flow cytometry. The mRNA levels of Treg-specific transcription factor Foxp3 in PBMCs, and PD-1 and PD-L1 in Treg cells were detected by qRT-PCR. The protein levels of PD-1 and PD-L1 in Treg cells were evaluated by western blot. The serum levels of TGF-β, IL-10, IL-35, and Foxp3 mRNA expression and CD4+CD25+ Treg cell percentage in PBMCs were decreased in PIH. Furthermore, a significant increase of PD-1 in Treg cells was found in PIH compared with NP. In addition, PD-L1 Fc, an activator of PD-1/PD-...Continue Reading

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Citations

Oct 5, 2019·American Journal of Reproductive Immunology : AJRI·Yang GuYuping Wang
Jan 19, 2020·European Journal of Immunology·Yong-Hong Zhang, Hai-Xiang Sun
Apr 9, 2020·Frontiers in Immunology·Yongbo ZhaoLiping Jin
Jul 20, 2019·Current Hypertension Reports·Tomasz P Mikolajczyk, Tomasz J Guzik
Jul 14, 2020·Frontiers in Immunology·Juliette KropMichael Eikmans
May 1, 2021·Rheumatology·Xia ZhangLiwei Lu

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Methods Mentioned

BETA
density gradient centrifugation
enzyme-linked immunosorbent assay
ELISA
flow cytometry
PCR
protein assay

Software Mentioned

CellQuest
Primer
Pro Plus
SPSS
Image
BLAST

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