PD-L1+ tumor-associated macrophages and PD-1+ tumor-infiltrating lymphocytes predict survival in primary testicular lymphoma

Haematologica
Marjukka PollariSirpa Leppä

Abstract

Primary testicular lymphoma is a rare and aggressive lymphoid malignancy, most often representing diffuse large B-cell lymphoma histologically. Tumor-associated macrophages and tumor-infiltrating lymphocytes have been associated with survival in diffuse large B-cell lymphoma, but their prognostic impact in primary testicular lymphoma is unknown. Here, we aimed to identify macrophages, their immunophenotypes and association with lymphocytes, and translate the findings into survival of patients with primary testicular lymphoma. We collected clinical data and tumor tissue from 74 primary testicular lymphoma patients, and used multiplex immunohistochemistry and digital image analysis to examine macrophage markers (CD68, CD163, and c-Maf), T-cell markers (CD3, CD4, and CD8), B-cell marker (CD20), and three checkpoint molecules (PD-L1, PD-L2, and PD-1). We demonstrate that a large proportion of macrophages (median 41%, range 0.08-99%) and lymphoma cells (median 34%, range 0.1-100%) express PD-L1. The quantity of PD-L1+ CD68+ macrophages correlates positively with the amount of PD-1+ lymphocytes, and a high proportion of either PD-L1+ CD68+ macrophages or PD-1+ CD4+ and PD-1+ CD8+ T cells translates into favorable survival. In contras...Continue Reading

Citations

Mar 21, 2020·Medicine·Qi WangYisheng Tao
Dec 8, 2019·Virchows Archiv : an International Journal of Pathology·Melissa AlameVanessa Szablewski
Jul 8, 2020·European Journal of Haematology·Marjukka PollariSirpa Leppä
Mar 24, 2020·Neuro-oncology Advances·Alexander OuAmy B Heimberger
Nov 30, 2019·Journal of Immunology Research·Di LuKaican Cai
Nov 19, 2019·Pathology·Narittee SukswaiL Jeffrey Medeiros
Nov 13, 2020·Journal of Immunology Research·John ApostolidisHani Al Hashmi
Oct 17, 2020·Leukemia & Lymphoma·Sheren Younes, Yasodha Natkunam
Aug 9, 2021·Drug Delivery and Translational Research·Anujan RameshAshish A Kulkarni
Aug 19, 2021·Journal of Hematology & Oncology·Yingyue LiuXin Wang
Jul 11, 2020·Cancer Cell·Olli DufvaSatu Mustjoki
Jul 4, 2021·Modern Pathology : an Official Journal of the United States and Canadian Academy of Pathology, Inc·Oscar BrückAnna Kreutzman

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