PD1hi cells associate with clusters of proliferating B-cells in marginal zone lymphoma

Diagnostic Pathology
Katherine WickendenMatthew J Ahearne

Abstract

Abnormally sustained immune reactions drive B-cell proliferation in some cases of marginal zone lymphoma but the CD4+ T-cell subsets, which are likely to contribute to the B-cell responses in the tumour microenvironment, are not well characterised and neither has the spatial distribution of the different subsets in involved lymph nodes been investigated. Employing a workflow of multiplex semi-automated immunohistochemistry combined with image processing we investigated association between infiltrating T-cells and proliferating lymphoma B-cells. Both total numbers of activating follicular helper (Tfh) cells (defined by high expression of PD1) and suppressive regulatory (Treg) T-cells (defined by FOXP3+ expression) and the Tfh:Treg ratio, assessed over relatively large areas of tissue, varied among cases of marginal zone lymphoma. We determined spatial distribution and demonstrated that PD1hi cells showed significantly more clustering than did FOXP3+. To investigate the association of infiltrating T-cells with lymphoma B-cells we employed Pearson correlation and Morisita-Horn index, statistical measures of interaction. We demonstrated that PD1hi cells were associated with proliferating B-cells and confirmed this by nearest neighb...Continue Reading

References

Oct 4, 2005·Proceedings of the National Academy of Sciences of the United States of America·Aravind SubramanianJill P Mesirov
Oct 13, 2006·Journal of Clinical Oncology : Official Journal of the American Society of Clinical Oncology·Abigail M LeeLindsey K Goff
Feb 19, 2009·The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society·Daniel PiriciRadu Stanescu
Apr 15, 2009·The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society·George GlassJames W Mandell
Jan 29, 2010·Seminars in Immunopathology·Michelle A Linterman, Carola G Vinuesa
Feb 10, 2010·The Journal of Experimental Medicine·Michelle A LintermanCarola G Vinuesa
Jul 26, 2011·Nature Medicine·Michelle A LintermanCarola G Vinuesa
Aug 30, 2012·Nature Methods·Caroline A SchneiderKevin W Eliceiri
Dec 13, 2012·Hematology·Pier Luigi Zinzani
Jun 20, 2013·The Journal of Clinical Investigation·Chunyan Gu-TrantienKaren Willard-Gallo
Jul 12, 2014·Virchows Archiv : an International Journal of Pathology·Matthew J AhearneSimon D Wagner
Oct 17, 2014·Clinical Cancer Research : an Official Journal of the American Association for Cancer Research·Emanuele ZuccaFranco Cavalli
Mar 18, 2015·Clinical Cancer Research : an Official Journal of the American Association for Cancer Research·Marina ParryJonathan C Strefford
Sep 24, 2015·Breast Cancer Research : BCR·Carlo C MaleyYinyin Yuan
Nov 26, 2015·Cancer Letters·Sidra Nawaz, Yinyin Yuan
Feb 26, 2016·Annual Review of Immunology·Carola G VinuesaIan C M MacLennan
Mar 19, 2016·Blood·Luca ArcainiMarco Paulli
Mar 16, 2017·Best Practice & Research. Clinical Haematology·Ming-Qing Du

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Datasets Mentioned

BETA
GSE32233
GSE32231

Methods Mentioned

BETA
biopsy

Software Mentioned

R
Visiopharm
GSEA
GraphPad
Prism
ggplot2
ImageJ
Spatstat

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