Abstract
Tissue remodeling of sub-epithelial mesenchymal cells is a major pathology occurring in chronic obstructive pulmonary disease (COPD) and asthma. Fibroblasts, as a major source of interstitial connective tissue extracellular matrix, contribute to the fibrotic and inflammatory changes in these airways diseases. Previously, we described that protein arginine methyltransferase-1 (PRMT1) participates in airway remodeling in a rat model of pulmonary inflammation. In this study we investigated the mechanism by which PDGF-BB regulates PRMT1 in primary lung fibroblasts, isolated from human lung biopsies. Fibroblasts were stimulated with PDGF-BB for up-to 48h and the regulatory and activation of signaling pathways controlling PRMT1 expression were determined. PRMT1 was localized by immuno-histochemistry in human lung tissue sections and by immunofluorescence in isolated fibroblasts. PRMT1 activity was suppressed by the pan-PRMT inhibitor AMI1. ERK1/2 mitogen activated protein kinase (MAPK) was blocked by PD98059, p38 MAPK by SB203580, and STAT1 by small interference (si) RNA treatment. The results showed that PDGF-BB significantly increased PRMT1 expression after 1h lasting over 48h, through ERK1/2 MAPK and STAT1 signaling. The inhibitio...Continue Reading
References
Feb 24, 2001·Transplantation·M TammA Glanville
Mar 27, 2001·Immunology and Cell Biology·D Knight
Jan 1, 2007·Recent Patents on Inflammation & Allergy Drug Discovery·Desideria DescalziG Walter Canonica
Mar 25, 2009·Clinical and Experimental Allergy : Journal of the British Society for Allergy and Clinical Immunology·M DegenR Chiquet-Ehrismann
Jun 4, 2010·The Clinical Respiratory Journal·Gunilla Westergren-ThorssonLeif Bjermer
Apr 18, 2012·Journal of Pineal Research·Almudena LalienaMaría J Tuñón
May 15, 2013·Fibrogenesis & Tissue Repair·Johanna DonovanDavid Abraham
Jun 19, 2013·Trends in Molecular Medicine·Yihai Cao
Jul 5, 2013·PloS One·Yanfen FangQiaojun He
Citations
Jan 13, 2017·Epigenetics : Official Journal of the DNA Methylation Society·Suzanne AcklooSusanne Müller
May 10, 2017·Oncogene·X W ChenJ Yang
Nov 16, 2018·Experimental Lung Research·Liuzhao CaoLiang Dong
Dec 12, 2018·The Journal of Immunology : Official Journal of the American Association of Immunologists·Qingzhu SunMichael Roth
Aug 31, 2019·The European Respiratory Journal·Qingzhu SunDaiana Stolz
Jan 26, 2020·International Journal of Molecular Sciences·Lei FangMichael Roth
Jan 12, 2017·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Farai GombedzaSailaja Paruchuri
Aug 19, 2018·Journal of Experimental & Clinical Cancer Research : CR·Yongkui LiLin Wang
Dec 5, 2019·Scientific Reports·Jana DietrichStefan Schrader
May 3, 2020·International Journal of Molecular Sciences·Eunji KimJae Youl Cho
Jul 27, 2017·Molecular and Cellular Biochemistry·Lei XiaZan-Dong Zhao
Jun 27, 2019·Scientific Reports·Jie ZhaoIrina Tikhanovich
May 28, 2019·Nature Communications·Yanpeng LiChiyu Zhang
Jun 5, 2020·Nature Communications·Lu CuiGerlinde Wernig
May 7, 2020·Scientific Reports·Akiko MammotoTadanori Mammoto
Dec 10, 2020·Pulmonary Circulation·Kang WuJason X-J Yuan
Sep 5, 2020·The European Respiratory Journal·Christopher D PascoeUNKNOWN Canadian Respiratory Research Network
Mar 12, 2021·International Journal of Oral Science·Bomiao CuiPing Zhang
Mar 21, 2021·Biochimica Et Biophysica Acta. Molecular Cell Research·Xiaozhen ZhangShemin Lu
Jan 14, 2017·The Journal of Allergy and Clinical Immunology·Qingzhu SunMichael Roth
Jun 21, 2021·Biochimica Et Biophysica Acta. Molecular Cell Research·Ai JinShemin Lu
Jul 3, 2021·International Journal of Molecular Sciences·Paula MonteroJulio Cortijo
Oct 12, 2017·Journal of Medicinal Chemistry·Chen WangCheng Luo
Aug 28, 2021·Respiratory Research·Huiyuan WangWei Hou
Aug 31, 2021·Frontiers in Endocrinology·Anqi ChenYanan Li
Sep 22, 2018·Gene Expression·Jie ZhaoIrina Tikhanovich