PDGF-induced phosphorylation of Tyr28 in the N-terminus of Fyn affects Fyn activation

Biochemical and Biophysical Research Communications
K HansenC H Heldin

Abstract

Binding of platelet-derived growth factor (PDGF) to its receptors leads to the activation of members of the Src family of protein tyrosine kinases. We show here that Fyn, a member of the Src family, is phosphorylated on Tyr28 in the unique N-terminal part of the molecule after interaction with the intracellular domain of the PDGF beta-receptor. Activated Fyn furthermore undergoes autophosphorylation on Tyr30, Tyr39 and Tyr420. When Fyn mutants with Tyr28, Tyr30 or Tyr39 replaced with phenylalanine residues were transfected into NIH3T3 cells a decreased activation after PDGF stimulation was seen, suggesting a functional importance of the N-terminal tyrosine phosphorylation of Fyn.

Citations

Jul 30, 2014·Frontiers in Genetics·Irene AmataMiquel Pons
Dec 13, 2005·Growth Factors·David A ClumpDaniel C Flynn
Feb 6, 2016·Oncotarget·Rohit A ChouguleLars Rönnstrand
Jun 21, 2007·The Journal of Immunology : Official Journal of the American Association of Immunologists·Ramarao VepacheduRafeul Alam
Jun 26, 2020·International Journal of Molecular Sciences·Carmela MatroneGabriella Ferretti
Jun 8, 2013·Chembiochem : a European Journal of Chemical Biology·Irene AmataMiquel Pons
Oct 31, 2020·Zeitschrift Für Naturforschung. C, a Journal of Biosciences·Deisy Perdomo, José Bubis
Sep 18, 1998·Biochimica Et Biophysica Acta·C H HeldinL Rönnstrand
Aug 26, 2021·Molecular Neurobiology·Bianca GugliettiLyndsey E Collins-Praino

❮ Previous
Next ❯

Related Concepts

Related Feeds

Cell Signaling by Tyrosine Kinases

Receptor tyrosine kinases (RTKs) are the high-affinity cell surface receptors for many polypeptide growth factors, cytokines, and hormones. RTKs have been shown not only to be key regulators of normal cellular processes but also to have a critical role in the development and progression of many types of cancer. Discover the latest research on cell signaling and RTK here.