PDGF-Ralpha gene expression predicts proliferation, but PDGF-A suppresses transdifferentiation of neonatal mouse lung myofibroblasts.

Respiratory Research
Patricia W KimaniStephen E McGowan

Abstract

Platelet-derived growth factor A (PDGF-A) signals solely through PDGF-Ralpha, and is required for fibroblast proliferation and transdifferentiation (fibroblast to myofibroblast conversion) during alveolar development, because pdgfa-null mice lack both myofibroblasts and alveoli. However, these PDGF-A-mediated mechanisms remain incompletely defined. At postnatal days 4 and 12 (P4 and P12), using mouse lung fibroblasts, we examined (a) how PDGF-Ralpha correlates with ki67 (proliferation marker) or alpha-smooth muscle actin (alphaSMA, myofibroblast marker) expression, and (b) whether PDGF-A directly affects alphaSMA or modifies stimulation by transforming growth factor beta (TGFbeta). Using flow cytometry we examined PDGF-Ralpha, alphaSMA and Ki67 in mice which express green fluorescent protein (GFP) as a marker for PDGF-Ralpha expression. Using real-time RT-PCR we quantified alphaSMA mRNA in cultured Mlg neonatal mouse lung fibroblasts after treatment with PDGF-A, and/or TGFbeta. The intensity of GFP-fluorescence enabled us to distinguish three groups of fibroblasts which exhibited absent, lower, or higher levels of PDGF-Ralpha. At P4, more of the higher than lower PDGF-Ralpha + fibroblasts contained Ki67 (Ki67+), and Ki67+ fibro...Continue Reading

References

Dec 1, 1978·The Anatomical Record·C Vaccaro, J S Brody
Aug 1, 1990·Differentiation; Research in Biological Diversity·K O LeslieR B Low
Oct 1, 1995·American Journal of Respiratory Cell and Molecular Biology·J C BonnerA R Osornio-Vargas
May 12, 1998·The American Journal of Physiology·C D Balnave, D G Allen
Sep 10, 1999·Arteriosclerosis, Thrombosis, and Vascular Biology·M B HautmannG K Owens
Feb 1, 2000·Journal of Cellular Physiology·T Scholzen, J Gerdes
Mar 17, 2001·Nature Reviews. Molecular Cell Biology·J Massagué
Dec 6, 2001·American Journal of Respiratory and Critical Care Medicine·D WarburtonB Driscoll
Dec 18, 2001·Mechanisms of Development·Karin AaseUlf Eriksson
Feb 16, 2002·Methods : a Companion to Methods in Enzymology·K J Livak, T D Schmittgen
May 4, 2002·Nature Reviews. Molecular Cell Biology·James J TomasekRobert A Brown
Aug 10, 2002·American Journal of Physiology. Lung Cellular and Molecular Physiology·F DemayoJ S Torday
Oct 12, 2002·American Journal of Physiology. Lung Cellular and Molecular Physiology·William M ManiscalcoColleen P Shea
Oct 25, 2002·Proceedings of the National Academy of Sciences of the United States of America·Da-Zhi WangEric N Olson
May 16, 2003·Molecular and Cellular Biology·T Guy HamiltonPhilippe Soriano
Jan 31, 2004·American Journal of Physiology. Heart and Circulatory Physiology·Frédéric Dandré, Gary K Owens
Mar 9, 2004·American Journal of Physiology. Lung Cellular and Molecular Physiology·Josephine HjobergEric S Silverman
Jun 23, 2004·Cytokine & Growth Factor Reviews·Christer Betsholtz
Jun 23, 2004·Cytokine & Growth Factor Reviews·James C Bonner
Dec 14, 2004·American Journal of Physiology. Lung Cellular and Molecular Physiology·Hui ChenWei Shi
Mar 9, 2005·Journal of Cellular Physiology·Y ArtemenkoA Sorisky
Dec 3, 2005·Genes & Development·Joan MassaguéDavid Wotton
Nov 7, 2006·American Journal of Physiology. Lung Cellular and Molecular Physiology·J S Torday, V K Rehan
Feb 17, 2007·Acta Pharmacologica Sinica·Li GuXin-Lun Tian
May 26, 2007·The American Journal of Pathology·Boris HinzGiulio Gabbiani
Oct 16, 2007·Chest·Chris J Scotton, Rachel C Chambers
Dec 21, 2007·Developmental Dynamics : an Official Publication of the American Association of Anatomists·Miguel A Alejandre-AlcázarRory E Morty
Mar 12, 2008·Laboratory Investigation; a Journal of Technical Methods and Pathology·Jianguo Lin, Anping Chen
May 17, 2008·Genes & Development·Johanna AndraeChrister Betsholtz
Oct 4, 2008·The Anatomical Record : Advances in Integrative Anatomy and Evolutionary Biology·Stephen E McGowanAmey J Holmes

❮ Previous
Next ❯

Citations

Jun 2, 2012·American Journal of Respiratory Cell and Molecular Biology·Leiling ChenAnne-Karina T Perl
Jun 11, 2011·Pediatric Research·Stephen E McGowan, Diann M McCoy
May 21, 2014·Proceedings of the National Academy of Sciences of the United States of America·Haruko Watanabe-TakanoMasahiko Hatano
Mar 19, 2014·Birth Defects Research. Part A, Clinical and Molecular Teratology·Stephen E McGowan
Sep 29, 2015·American Journal of Respiratory Cell and Molecular Biology·Jenna GreenAnne-Karina T Perl
Oct 11, 2014·American Journal of Respiratory Cell and Molecular Biology·Nina NoskovičováKatharina Heinzelmann
Jan 10, 2018·American Journal of Respiratory Cell and Molecular Biology·Matthew D McGrawLivia A Veress
Mar 16, 2018·American Journal of Physiology. Lung Cellular and Molecular Physiology·Stephen E McGowan, Diann M McCoy
Oct 27, 2017·European Respiratory Review : an Official Journal of the European Respiratory Society·Rana Kanaan, Charlie Strange
Jul 4, 2015·American Journal of Physiology. Lung Cellular and Molecular Physiology·Stephen E McGowan, Diann M McCoy
Aug 26, 2014·American Journal of Physiology. Lung Cellular and Molecular Physiology·Stephen E McGowan, Diann M McCoy
Aug 5, 2017·American Journal of Physiology. Lung Cellular and Molecular Physiology·Stephen E McGowan, Diann M McCoy
Sep 1, 2015·American Journal of Physiology. Lung Cellular and Molecular Physiology·Aglaia NtokouKatrin Ahlbrecht
Sep 2, 2017·American Journal of Respiratory Cell and Molecular Biology·Shawn K Ahlfeld, Anne Karina Perl
Sep 6, 2015·American Journal of Physiology. Lung Cellular and Molecular Physiology·Jordi Ruiz-Camp, Rory E Morty
Aug 13, 2013·American Journal of Physiology. Lung Cellular and Molecular Physiology·Patricia R BachillerJesse D Roberts
Jun 12, 2013·American Journal of Physiology. Lung Cellular and Molecular Physiology·Stephen E McGowan, Diann M McCoy
Mar 26, 2013·Developmental Dynamics : an Official Publication of the American Association of Anatomists·Shubing ZhangKeli Xu
Nov 17, 2020·Journal of the American Heart Association·Laura E BruijnJan H N Lindeman
Dec 9, 2021·American Journal of Physiology. Lung Cellular and Molecular Physiology·Ying ZhongJesse D Roberts

❮ Previous
Next ❯

Methods Mentioned

BETA
flow cytometry
PCR
fluorescence microscopy
scraping
Confocal
Assay
FACS
nuclear translocation
flow
SMA

Software Mentioned

Excel
Sigma Stat
IP lab

Related Concepts

Related Feeds

CFTR Mutant Structural Therapy

Over 1700 different mutations in the CFTR genes have been shown to cause cystic fibrosis. Here is the latest research on structural therapy for CFTR mutants.

Related Papers

Zhongguo xiu fu chong jian wai ke za zhi = Zhongguo xiufu chongjian waike zazhi = Chinese journal of reparative and reconstructive surgery
H HuangW H Bao
Zhonghua lao dong wei sheng zhi ye bing za zhi = Zhonghua laodong weisheng zhiyebing zazhi = Chinese journal of industrial hygiene and occupational diseases
Yong-bin HuJi-fang Wen
© 2022 Meta ULC. All rights reserved