PMID: 9660860Jul 14, 1998Paper

PDMP blocks brefeldin A-induced retrograde membrane transport from golgi to ER: evidence for involvement of calcium homeostasis and dissociation from sphingolipid metabolism

The Journal of Cell Biology
J W KokD Hoekstra

Abstract

In this study, we show that an inhibitor of sphingolipid biosynthesis, D,L-threo-1-phenyl-2- decanoylamino-3-morpholino-1-propanol (PDMP), inhibits brefeldin A (BFA)-induced retrograde membrane transport from Golgi to endoplasmic reticulum (ER). If BFA treatment was combined with or preceded by PDMP administration to cells, disappearance of discrete Golgi structures did not occur. However, when BFA was allowed to exert its effect before PDMP addition, PDMP could not "rescue" the Golgi compartment. Evidence is presented showing that this action of PDMP is indirect, which means that the direct target is not sphingolipid metabolism at the Golgi apparatus. A fluorescent analogue of PDMP, 6-(N-[7-nitro-2,1, 3-benzoxadiazol-4-yl]amino)hexanoyl-PDMP (C6-NBD-PDMP), did not localize in the Golgi apparatus. Moreover, the effect of PDMP on membrane flow did not correlate with impaired C6-NBD-sphingomyelin biosynthesis and was not mimicked by exogenous C6-ceramide addition or counteracted by exogenous C6-glucosylceramide addition. On the other hand, the PDMP effect was mimicked by the multidrug resistance protein inhibitor MK571. The effect of PDMP on membrane transport correlated with modulation of calcium homeostasis, which occurred in a...Continue Reading

References

Jul 15, 1992·Proceedings of the National Academy of Sciences of the United States of America·J G DonaldsonR D Klausner
Jan 1, 1992·The Journal of Cell Biology·J AlcaldeI V Sandoval
Mar 1, 1992·The Journal of Cell Biology·R D KlausnerJ Lippincott-Schwartz
Dec 24, 1991·Biochemistry·J C McIntyre, R G Sleight
Feb 1, 1991·The Journal of Cell Biology·J Lippincott-SchwartzR D Klausner
May 10, 1985·Science·N G Lipsky, R E Pagano
Mar 8, 1995·Biochemical and Biophysical Research Communications·V GekelerJ Beck
Feb 1, 1994·The Journal of Cell Biology·Z ElazarJ E Rothman
Dec 1, 1993·The Journal of Cell Biology·G TanigawaJ E Rothman
Jun 1, 1993·Pflügers Archiv : European journal of physiology·R NitschkeR Greger
Jul 5, 1996·The Journal of Biological Chemistry·W NickelF T Wieland
Aug 9, 1996·The Journal of Biological Chemistry·Y LavieM C Cabot
Apr 1, 1997·Cell Biology International·W Zhang, G M Roomans
Aug 1, 1997·The Journal of Biological Chemistry·L JohannesB Goud
Jan 7, 1998·The Journal of Cell Biology·A MironovA Luini
Jan 7, 1998·The Journal of Cell Biology·N SciakyJ Lippincott-Schwartz
Feb 12, 1998·European Journal of Pharmacology·C M FilipeanuS A Nelemans
Feb 14, 1998·The Journal of Cell Biology·N B ColeJ Lippincott-Schwartz
Aug 1, 1959·Canadian Journal of Biochemistry and Physiology·E G BLIGH, W J DYER
Aug 1, 1996·Trends in Cell Biology·M Aridor, W E Balch
Dec 1, 1996·Trends in Cell Biology·S Y BednarekR Schekman

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Citations

Aug 11, 2007·Cancer Chemotherapy and Pharmacology·Shirley Cruz LioMark L Richards
Jul 3, 2002·Chemistry and Physics of Lipids·Olaf MaierDick Hoekstra
Jun 20, 2001·European Journal of Pharmacology·C M FilipeanuS A Nelemans
Jun 29, 2002·Breast Cancer Research and Treatment·David ChauvierMichel Manfait
May 21, 1999·European Journal of Biochemistry·E LatorreR E Catalán
Aug 6, 1999·Molecular Biology of the Cell·S D Conner, G M Wessel
Dec 12, 2012·Journal of Experimental Botany·Falco KrügerDavid G Robinson
Jul 31, 2013·PloS One·Matti A Kjellberg, Peter Mattjus
Feb 22, 2002·Biochemical and Biophysical Research Communications·Akira TakatsukiYoshiki Kono
Jun 1, 2000·FEBS Letters·C M FilipeanuJ W Kok
Nov 21, 2015·Current Tropical Medicine Reports·Tavis L MendezSiddhartha Das
Apr 30, 2009·Journal of Cell Science·Takayuki IinumaKatsuko Tani
Jul 19, 2006·Journal of Cell Science·Teresa SprocatiNica Borgese

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