Pdr18 is involved in yeast response to acetic acid stress counteracting the decrease of plasma membrane ergosterol content and order

Scientific Reports
Cláudia P GodinhoIsabel Sá-Correia

Abstract

Saccharomyces cerevisiae has the ability to become less sensitive to a broad range of chemically and functionally unrelated cytotoxic compounds. Among multistress resistance mechanisms is the one mediated by plasma membrane efflux pump proteins belonging to the ABC superfamily, questionably proposed to enhance the kinetics of extrusion of all these compounds. This study provides new insights into the biological role and impact in yeast response to acetic acid stress of the multistress resistance determinant Pdr18 proposed to mediate ergosterol incorporation in plasma membrane. The described coordinated activation of the transcription of PDR18 and of several ergosterol biosynthetic genes (ERG2-4, ERG6, ERG24) during the period of adaptation to acetic acid inhibited growth provides further support to the involvement of Pdr18 in yeast response to maintain plasma membrane ergosterol content in stressed cells. Pdr18 role in ergosterol homeostasis helps the cell to counteract acetic acid-induced decrease of plasma membrane lipid order, increase of the non-specific membrane permeability and decrease of transmembrane electrochemical potential. Collectively, our results support the notion that Pdr18-mediated multistress resistance is cl...Continue Reading

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Citations

Nov 7, 2018·Applied Microbiology and Biotechnology·Joana T CunhaLucília Domingues
Jun 20, 2019·Scientific Reports·Anna Ligasová, Karel Koberna
Feb 2, 2019·Folia Microbiologica·Alexandra BencovaYvetta Gbelska
Sep 29, 2019·Research in Microbiology·Alexis MorenoPierre Falson
Aug 17, 2020·Journal of Microbiological Methods·V M GrechkoV T Cheshchevik
Mar 7, 2020·Food Microbiology·Juan A Porras-AgüeraTeresa García-Martínez
Mar 7, 2021·Fungal Genetics and Biology : FG & B·Sonam KumariRajendra Prasad
May 6, 2021·International Journal of Molecular Sciences·Narakorn Khunweeraphong, Karl Kuchler
May 18, 2021·Yeast·Nicoletta Guaragnella, Maurizio Bettiga
Jan 28, 2020·Journal of Agricultural and Food Chemistry·Yun DuMu Li

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Methods Mentioned

BETA
fluorescence microscopy

Software Mentioned

MetaMorph
MATLAB
Primer Express

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