PDX-1 interaction and regulation of the Pancreatic Derived Factor (PANDER, FAM3B) promoter.

Biochimica Et Biophysica Acta
Brant R BurkhardtBryan A Wolf

Abstract

Pancreatic Derived Factor (PANDER) is a novel cytokine-like protein dominantly expressed within the endocrine pancreas. Our previous study demonstrated that the PANDER promoter was both tissue-specific and glucose-responsive. Surrounding the PANDER transcriptional start site are several putative A- and E-Box elements that may bind to the various pancreatic transcriptional factors of MafA, BETA2/NeuroD, and Pancreatic Duodenal Homeobox-1 (PDX-1). To characterize the transcriptional regulatory factors involved in PANDER gene expression, we performed co-transfection reporter gene analysis and demonstrated upregulation by all three transcription factors, with the greatest individual increase stemming from PDX-1. Potential binding of PDX-1 to A box (TAAT) regions of the PANDER promoter was demonstrated by chromatin immunoprecipitation (ChIP) and further corroborated by electrophoretic mobility shift assay (EMSA). Binding of PDX-1 to the A box regions was inhibited by mutagenized (TAGT) oligonucleotides. Site-directed mutagenesis of the three PDX-1 A box binding motifs revealed that A box sites 2 and 3 in combination were critical for maximal gene expression and deletion resulted in a 82% reduction in promoter activity. Furthermore, ...Continue Reading

Citations

Nov 30, 2010·International Journal of Endocrinology·Chunjiong WangJichun Yang
Jun 15, 2011·FEBS Letters·Camella G WilsonBrant R Burkhardt
Jul 20, 2010·Molecular and Cellular Endocrinology·Jason R CarnegieBrant R Burkhardt
Apr 9, 2013·Biochimica Et Biophysica Acta·Yanlan ZhouJichun Yang
Jul 1, 2015·Molecular and Cellular Endocrinology·Whitney A RatliffBrant R Burkhardt
Jul 24, 2013·Journal of Gastroenterology and Hepatology·Jichun Yang, Youfei Guan
Jun 15, 2011·Molecular and Cellular Endocrinology·Xinwei HouYingying Le
Jul 11, 2016·Molecular and Cellular Endocrinology·Mark G AthanasonBrant R Burkhardt
Jan 29, 2014·The Journal of Endocrinology·Claudia E Robert-CoopermanBrant R Burkhardt
Sep 14, 2019·Journal of Receptor and Signal Transduction Research·Goverdhan LankaSarita Rajender Potlapally
Aug 6, 2021·Expert Opinion on Pharmacotherapy·Marc S Rendell

❮ Previous
Next ❯

Related Concepts

Related Feeds