PDZ-scaffold protein, Tamalin promotes dendritic outgrowth and arborization in rat hippocampal neuron

Biochemical and Biophysical Research Communications
Jiwon MoHyun Kim

Abstract

Tamalin is a scaffold protein known to regulate membrane trafficking through its interaction with cytohesin-2/ARNO, guanine nucleotide exchange factor (GEF) on ADP-ribosylation factor (Arf) 1/6, and induces actin reorganization. However, the neuronal function of Tamalin is not well understood. Here, we report that Tamalin participates in neurite development through the association with exchange factor for Arf6 (EFA6A)/Arf6 signaling. In immature hippocampal neuron, Tamalin knockdown markedly reduced the dendritic outgrowth, the number of dendritic tips and the levels of filamentous actin (F-actin) and microtubule-associated protein 2 (MAP2) in dendrites. In addition, Tamalin colocalized with EFA6A and Arf6 in the dendritic shaft. Tamalin knockdown reduced the number, size, and intensity of endogenous EFA6A cluster, whereas overexpression of Tamalin showed opposite effects compared with those of knockdown. These results suggest that Tamalin is responsible for neuronal dendritic development via regulation of EFA6A/Arf6-mediated cytoskeleton dynamics.

References

Mar 22, 2000·Proceedings of the National Academy of Sciences of the United States of America·P A RandazzoJ A Cooper
Aug 7, 2002·The Journal of Cell Biology·Akihiro HaradaNobutaka Hirokawa
Jan 20, 2004·Nature Neuroscience·Barbara F AkumBonnie L Firestein
Jun 3, 2004·The Journal of Biological Chemistry·Masayuki HiroseShigetada Nakanishi
Sep 21, 2004·Nature Reviews. Neuroscience·Eunjoon Kim, Morgan Sheng
Apr 25, 2006·Nature Reviews. Molecular Cell Biology·Crislyn D'Souza-Schorey, Philippe Chavrier
Apr 26, 2006·The Journal of Cell Biology·Pedro F EstebanLino Tessarollo
May 5, 2006·The Journal of Neuroscience : the Official Journal of the Society for Neuroscience·Seungwon ChoiEunjoon Kim
Oct 6, 2006·The Journal of Neuroscience : the Official Journal of the Society for Neuroscience·Erik I CharychBonnie L Firestein
Oct 20, 2006·The Journal of Neuroscience : the Official Journal of the Society for Neuroscience·Mitsuko Watabe-UchidaLinda Van Aelst
Jun 6, 2009·Journal of Cell Science·Cristina SironiDaniela Talarico
Dec 17, 2009·Molecular and Cellular Biology·Ayuko SakaneTakuya Sasaki
Dec 17, 2010·The Journal of Neuroscience : the Official Journal of the Society for Neuroscience·Carlos J Ballester-RosadoHui-Chen Lu
Aug 10, 2011·Proceedings of the National Academy of Sciences of the United States of America·Ragna SannerudWim Annaert
Mar 8, 2012·The Journal of Neuroscience : the Official Journal of the Society for Neuroscience·Sudhirkumar U YanpallewarLino Tessarollo

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Citations

Sep 24, 2013·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Hei Nga Maggie CheungKwok-Fai Lau
Jan 11, 2014·Photochemical & Photobiological Sciences : Official Journal of the European Photochemistry Association and the European Society for Photobiology·Anand VenkataramanMark Leid
Jun 16, 2018·Neurology. Genetics·John K L WongStacey S Cherny

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