PDZD8 interacts with Protrudin and Rab7 at ER-late endosome membrane contact sites associated with mitochondria.

Nature Communications
Yael Elbaz-AlonJodi Nunnari

Abstract

Endosomes are compositionally dynamic organelles that regulate signaling, nutrient status and organelle quality by specifying whether material entering the cells will be shuttled back to the cell surface or degraded by the lysosome. Recently, membrane contact sites (MCSs) between the endoplasmic reticulum (ER) and endosomes have emerged as important players in endosomal protein sorting, dynamics and motility. Here, we show that PDZD8, a Synaptotagmin-like Mitochondrial lipid-binding Proteins (SMP) domain-containing ER transmembrane protein, utilizes distinct domains to interact with Rab7-GTP and the ER transmembrane protein Protrudin and together these components localize to an ER-late endosome MCS. At these ER-late endosome MCSs, mitochondria are also recruited to form a three-way contact. Thus, our data indicate that PDZD8 is a shared component of two distinct MCSs and suggest a role for SMP-mediated lipid transport in the regulation of endosome function.

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Citations

Jan 13, 2021·Developmental Cell·Haoxi Wu, Gia K Voeltz
Jan 2, 2021·Frontiers in Cell and Developmental Biology·Michiko Shirane
Jan 30, 2021·Frontiers in Cell and Developmental Biology·Aurora Gil-HernándezAlejandro Silva-Palacios
Jan 16, 2021·Trends in Cell Biology·Luis-Carlos TábaraJulien Prudent
Mar 7, 2021·International Journal of Molecular Sciences·Veselina PetrovaRichard Eva
Apr 17, 2021·Genes & Development·Maja PetkovicYuh Nung Jan
Apr 21, 2021·BioFactors·Plácido NavasCarlos Santos-Ocaña
Jun 8, 2021·Frontiers in Cell and Developmental Biology·Saeko Aoyama-Ishiwatari, Yusuke Hirabayashi
Jul 9, 2021·Cell Host & Microbe·Philipp WalchAthanasios Typas
Jul 25, 2021·Nature Communications·Nazmiye ÖzkanGinny G Farías
Aug 31, 2021·Clinical and Translational Medicine·Liyang LiXiangdong Wang
Sep 24, 2021·Scientific Reports·Haider KhanYoung Jun Im
Oct 26, 2021·Experimental Neurology·Eva M SzegöBjörn H Falkenburger

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Datasets Mentioned

BETA
PXD015523

Methods Mentioned

BETA
nucleotide exchange
immunoprecipitation
co-immunoprecipitation
immunoprecipitations
electron microscopy
fluorescence microscopy
transfection
transfections
protein assay
pull-down

Software Mentioned

VisView
Perseus
Xcalibur
CLEM
ProteomeXchange
ImageJ
SerialEM
MaxQuant
Icy
Andromeda

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