PE11, a PE/PPE family protein of Mycobacterium tuberculosis is involved in cell wall remodeling and virulence

Scientific Reports
Parul SinghSangita Mukhopadhyay

Abstract

The role of the unique proline-glutamic acid (PE)/proline-proline-glutamic acid (PPE) family of proteins in the pathophysiology and virulence of Mycobacterium tuberculosis is not clearly understood. One of the PE family proteins, PE11 (LipX or Rv1169c), specific to pathogenic mycobacteria is found to be over-expressed during infection of macrophages and in active TB patients. In this study, we report that M. smegmatis expressing PE11 (Msmeg-PE11) exhibited altered colony morphology and cell wall lipid composition leading to a marked increase in resistance against various environmental stressors and antibiotics. The cell envelope of Msmeg-PE11 also had greater amount of glycolipids and polar lipids. Msmeg-PE11 was found to have better survival rate in infected macrophages. Mice infected with Msmeg-PE11 had higher bacterial load, showed exacerbated organ pathology and mortality. The liver and lung of Msmeg-PE11-infected mice also had higher levels of IL-10, IL-4 and TNF-α cytokines, indicating a potential role of this protein in mycobacterial virulence.

References

Mar 1, 1995·Antimicrobial Agents and Chemotherapy·L DengM R McNeil
Oct 15, 1994·FEMS Microbiology Letters·V Jarlier, H Nikaido
Jul 14, 2000·Journal of Bacteriology·J RechtR Kolter
Jul 24, 2001·The Journal of Immunology : Official Journal of the American Association of Immunologists·G T Seah, G A Rook
Jan 5, 2002·The Journal of Immunology : Official Journal of the American Association of Immunologists·Hubert M TseAndrea M Cooper
Feb 13, 2003·Biotechnology and Applied Biochemistry·Rani GuptaSapna Bradoo
Mar 27, 2003·Molecular Microbiology·Christopher M SassettiEric J Rubin
Sep 4, 2003·The Journal of Experimental Medicine·Dirk SchnappingerGary K Schoolnik
Jun 9, 2005·Protein Expression and Purification·Min ZhangHong-hai Wang
Dec 6, 2005·Cell·María Mercedes Zambrano, Roberto Kolter
Dec 31, 2005·Journal of Bacteriology·Jeffrey M ChenJun Liu
Jan 24, 2006·Infection and Immunity·Helmy RachmanStefan H E Kaufmann
Sep 1, 2006·Molecular Microbiology·Ashwini ChauhanMalini Rajagopalan
Nov 23, 2006·Applied and Environmental Microbiology·Efstathios S GiotisBrian J Wilkinson
Aug 10, 2007·Clinical and Vaccine Immunology : CVI·Yeddula NarayanaKithiganahalli N Balaji
Jan 31, 2008·PloS One·Tige R RustadDavid R Sherman
Feb 20, 2009·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Nicholas P WestWarwick J Britton
Mar 11, 2009·Microbial Pathogenesis·Ute SchwabDavid G Russell
May 27, 2010·Critical Reviews in Microbiology·Gurdyal SinghJagdeep Kaur
Jul 14, 2010·Proceedings of the National Academy of Sciences of the United States of America·Eszter Lázár-MolnárWilliam R Jacobs
Feb 15, 2011·Clinical & Developmental Immunology·Samantha L Sampson
Jul 8, 2011·Frontiers in Microbiology·Martin I VoskuilGary K Schoolnik
Nov 1, 2011·Infection and Immunity·Guomiao ShenSuman Laal

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Citations

Sep 14, 2016·International Journal of Molecular Sciences·Ze-Jia CuiQing-Ye Zhang
Feb 7, 2017·Microbiological Research·Chunyan LiJianping Xie
Aug 23, 2017·Journal of Interferon & Cytokine Research : the Official Journal of the International Society for Interferon and Cytokine Research·Guoping YangLang Bao
Sep 27, 2018·Future Microbiology·Nagender Rao RameshwaramSangita Mukhopadhyay
May 8, 2020·Microbial Drug Resistance : MDR : Mechanisms, Epidemiology, and Disease·Ana Laura Granados-TristánKatia Peñuelas-Urquides
Oct 19, 2019·International Microbiology : the Official Journal of the Spanish Society for Microbiology·Sharda SharmaZeeshan Fatima
Jun 28, 2018·Pathogens and Disease·Jerome PrusaChristina L Stallings
Jun 6, 2020·International Journal of Molecular Sciences·Angel Ly, Jun Liu
May 27, 2020·BMC Microbiology·Ana Leticia Arriaga-GuerreroKatia Peñuelas-Urquides
Oct 12, 2018·Drug Development Research·Erik M Larsen, R Jeremy Johnson
Oct 13, 2020·Frontiers in Cellular and Infection Microbiology·Tarina SharmaSeyed Ehtesham Hasnain
Mar 18, 2021·International Journal of Medical Microbiology : IJMM·Aquib EhtramSeyed E Hasnain
May 1, 2021·Frontiers in Cellular and Infection Microbiology·Siyuan FengXi Huang
Jun 25, 2021·Biochemical and Biophysical Research Communications·Shruti Srivastava, Sangita Mukhopadhyay

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Methods Mentioned

BETA
transmission electron microscopy
esterase assay
glycosylation
environmental stress
PCR
Assay

Software Mentioned

ImageJ
GraphPad
GraphPad Prism

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