Peak dispersion and contributions to plate height in nonaqueous capillary electrophoresis at high electric field strengths: propanol as background electrolyte solvent

Electrophoresis
S PalonenMarja-Liisa Riekkola

Abstract

Peak dispersion effects in nonaqueous capillary electrophoretic separations of aromatic anionic analytes were investigated in a propanolic background electrolyte solution. Poly(glycidylmethacrylate-co-N-vinylpyrrolidone) coating was applied to the capillary to suppress the electroosmotic flow and to improve the repeatability of the migration times. Electrical field strengths up to 2000 Vcm(-1) were applied in separations and the separation efficiencies were compared with theoretical values calculated on the basis of plate height theory. The contributions to the total plate height were calculated for injection plug length, diffusion, Joule heating, electromigration dispersion, analyte adsorption to the capillary wall, and detector slit aperture length. Analyte diffusion coefficients were measured by Taylor dispersion method, while distribution constants were measured chromatographically. Agreement between the calculated and empirical results was fairly good even though some approximations were required. In most cases the longitudinal diffusion contribution governed the total plate height, while the contribution of Joule heating was insignificant even at exceptionally high field strengths used. The relatively long detection slit ...Continue Reading

Citations

Dec 30, 2004·Electrophoresis·Xiangchun Xuan, Dongqing Li
Jun 25, 2004·Journal of Chromatography. a·Simo P Porras, Ernst Kenndler
Dec 7, 2007·Electrophoresis·Xiangchun Xuan
Aug 31, 2017·Journal of Chromatography. a·Seung Jae LeeAndreas Manz

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