PMID: 11325423Apr 28, 2001Paper

Peanut (Arachis hypogaea) lectin recognizes alpha-linked galactose, but not N-acetyl lactosamine in N-linked oligosaccharide terminals

International Journal of Biological Macromolecules
B K Chacko, P S Appukuttan

Abstract

Peanut (Arachis hypogaea) agglutinin (PNA) is extensively used as tumour marker as it strongly recognises the cancer specific T antigen (Galbeta1-->3GalNAc-), but not its sialylated version. However, an additional specificity towards Galbeta1-->4GlcNAc (LacNAc), which is not tumour specific, had been attributed to PNA. For correct interpretation of lectin histochemical results we examined PNA sugar specificity using naturally occurring or semi-synthetic glycoproteins, matrix-immobilised galactosides and lectin-binding tissue glycoproteins, rather than mono- or disaccharides as ligands. Dot-blots, transfer blots or polystyrene plate coatings of the soluble glycoconjugates were probed with horse-radish peroxidase (HRP) conjugates of PNA and other lectins of known specificity. Modifications of PNA-binding glycoproteins, including selective removal of O-linked oligosaccharides and treatment with glycosidases revealed that Galbeta1-->4GlcNAc (LacNAc) was ineffective while terminal alpha-linked galactose (TAG) as well as exposed T antigen (Galbeta1-->3 GalNAc-) was excellent as sugar moiety in glycoproteins for their recognition by PNA. When immobilised, melibiose was superior to lactose in PNA binding. Results were confirmed using T...Continue Reading

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Citations

May 5, 2005·Experimental Cell Research·Robert C De Lisle
Mar 16, 2006·Proceedings of the National Academy of Sciences of the United States of America·François FoulquierGert Matthijs
Apr 9, 2009·Journal of Biomedical Materials Research. Part a·Sucharita P ShankarJulia E Babensee
Jan 7, 2009·Analytical Biochemistry·Thomas IskratschIain B H Wilson
Jul 6, 2004·The Journal of Comparative Neurology·Nicholas J GibsonLeslie P Tolbert
Jun 20, 2007·Microscopy Research and Technique·Laura Gómez-SantosJuan Francisco Madrid
Sep 13, 2017·PeerJ·Onn Haji HashimCheng-Siang Lee
Mar 3, 2007·Human Molecular Genetics·Christian KranzHudson H Freeze
Jun 14, 2018·Glycobiology·Shigeko Kijimoto-OchiaiToshiaki Koda
Feb 6, 2020·BMC Bioinformatics·Lachlan CoffAndrew J Guy
Mar 15, 2019·Emerging Microbes & Infections·Jimmy Chun Cheong LaiJohn M Nicholls
Mar 19, 2021·Biochemistry and Biophysics Reports·Lucía BidondoCecilia Giacomini
Dec 31, 2002·Biochemical and Biophysical Research Communications·W Michael SchopperleWilliam C DeWolf
Mar 22, 2012·Bioconjugate Chemistry·Francisco Javier Lopez-JaramilloFrancisco Santoyo-Gonzalez

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