Pectate lyase gene expression and enzyme activity in ripening banana fruit

Plant Molecular Biology
M Celia Marín-RodríguezGraham B Seymour

Abstract

Two distinct cDNA clones showing sequence homology to higher-plant pectate lyase (Pel) genes were isolated from ripening banana fruits. The transcripts were detected only in fruit tissue and both were strongly ripening-related. Yeast transformation with the most highly expressed Pel clone produced a recombinant protein with pectate lyase activity, demonstrating that this sequence was likely to encode a pectate lyase protein in planta. An assay developed for measuring the action of the endogenous enzyme from banana pulp tissue revealed a significant increase in calcium-dependent pectate lyase activity during ripening. The enhanced levels of enzyme activity corresponded with an increase in soluble polyuronides from banana pulp.

Citations

Mar 10, 2007·The Plant Journal : for Cell and Molecular Biology·Radnaa NaranAndrew J Mort
May 11, 2017·BMC Plant Biology·Roneel PrakashRoswitha Schröder
Apr 10, 2009·Journal of Experimental Botany·D Mbéguié-A-MbéguiéS Sidibé-Bocs
Dec 22, 2012·BMC Plant Biology·João Paulo FabiJoão Roberto Oliveira do Nascimento
Apr 24, 2012·Yi chuan = Hereditas·Ju-Hua LiuZhi-Qiang Jin
Apr 1, 2009·Physiology and Molecular Biology of Plants : an International Journal of Functional Plant Biology·Anurag PayasiRandhir Singh
Nov 13, 2004·The Journal of Biological Chemistry·Edmund W CzerwinskiRandall M Goldblum
Jul 28, 2016·Nature Biotechnology·Selman UluisikGraham B Seymour
Feb 13, 2018·Trends in Plant Science·Duoduo WangGraham B Seymour
Mar 24, 2006·Phytochemistry·Anurag PayasiGirdhar G Sanwal

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