PMID: 11903968Mar 21, 2002Paper

Pectin-bound beta-galactosidase present in cell walls of carrot cells under the different calcium status

Physiologia Plantarum
Haruyoshi KonnoKenji Katoh

Abstract

Glycosyl-hydrolytic enzymes from suspension-cultured carrot (Daucus carota L. cv. Kintoki) cells grown in calcium (Ca2+)-deficient and normal liquid media were studied after extraction successively by K-phosphate (pH 7.0) and Na-acetate (pH 5.2) containing 3 M LiCl. The same activities were detected in two protein fractions from control and Ca2+-deprived cells. The specific activities of alpha-galactosidase and polygalacturonase decreased under Ca2+ deprivation, but beta-galactosidase activity in the buffer-soluble protein from Ca2+-deprived cells increased 1.7-fold compared to control cells. Upon ion exchange and size-exclusion chromatography the fraction (Ca-Ia-I) in the buffer-soluble protein from Ca2+-deprived cells represented beta-galactosidase activity associated with a galacturonic acid-rich polysaccharide peak, whereas the corresponding fraction could hardly be detected in the buffer-soluble protein from control cells. Several of the same glycosidase activities were detected in the extract solubilized with cyclohexane-trans-1,2-diaminetetra-acetate (CDTA) from active cell walls of Ca2+-deprived cells as in the extract of control cells, but the beta-galactosidase activity was considerably reduced under Ca2+ deprivation....Continue Reading

Citations

Nov 3, 2009·Phytochemistry·Heather A Currie, Carole C Perry
Jun 2, 2005·Journal of Experimental Botany·Haruyoshi KonnoKenji Katoh
Feb 13, 2003·The Plant Journal : for Cell and Molecular Biology·Lesley McCartneyJ Paul Knox
Aug 18, 2020·Protein and Peptide Letters·Yuan-Yuan HanJosef Voglmeir
Aug 18, 2005·Journal of Agricultural and Food Chemistry·Malgorzata BaranskaLars P Christensen
Oct 20, 2009·Carbohydrate Research·Elena A GünterYury S Ovodov

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