Nov 5, 2018

Pectin digestion in herbivorous beetles: Impact of pseudoenzymes exceeds that of their active counterparts

BioRxiv : the Preprint Server for Biology
Roy KirschYannick Pauchet

Abstract

Many protein families harbor pseudoenzymes that have lost the catalytic function of their enzymatically active counterparts. Assigning alternative function and importance to these proteins is challenging. Because the evolution towards pseudoenzymes is driven by gene duplication, they often accumulate in multigene families. Plant cell wall-degrading enzymes (PCWDEs) are prominent examples of expanded gene families. The pectolytic glycoside hydrolase family 28 (GH28) allows herbivorous insects to break down the PCW polysaccharide pectin. GH28 in the Phytophaga clade of beetles contains many active enzymes but also many inactive counterparts. Using functional characterization, gene silencing, global transcriptome analyses and recordings of life history traits, we found that not only catalytically active but also inactive GH28 proteins are part of the same pectin-digesting pathway. The robustness and plasticity of this pathway and thus its importance for the beetle is supported by extremely high steady-state expression levels and counter-regulatory mechanisms. Unexpectedly, the impact of pseudoenzymes on the pectin-digesting pathway in Phytophaga beetles exceeds even the influence of their active counterparts, such as a lowered eff...Continue Reading

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Mentioned in this Paper

Biochemical Pathway
Multigene Family
Genes
Enzymes, antithrombotic
Food
Digests
Order Coleoptera
Cardiac Glycosides
Enzymes for Treatment of Wounds and Ulcers
Hydrolase

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