Pectin induced transcriptome of a Rhizoctonia solani strain causing sheath blight disease in rice reveals insights on key genes and RNAi machinery for development of pathogen derived resistance

Plant Molecular Biology
Talluri Bhaskar RaoSatendra K Mangrauthia

Abstract

RNAi mediated silencing of pectin degrading enzyme of R. solani gives a high level of resistance against sheath blight disease of rice. Rice sheath blight disease caused by Rhizoctonia solani Kuhn (telemorph; Thanatephorus cucumeris) is one of the most devastating fungal diseases which cause severe loss to rice grain production. In the absence of resistant cultivars, the disease is currently managed through fungicides which add to environmental pollution. To explore the potential of utilizing RNA interference (RNAi)-mediated resistance against sheath blight disease, we identified genes encoding proteins and enzymes involved in the RNAi pathway in this fungal pathogen. The RNAi target genes were deciphered by RNAseq analysis of a highly virulent strain of the R. solani grown in pectin medium. Additionally, pectin metabolism associated genes of R. solani were analyzed through transcriptome sequencing of infected rice tissues obtained from six diverse rice cultivars. One of the key candidate gene AG1IA_04727 encoding polygalacturonase (PG), which was observed to be significantly upregulated during infection, was targeted through RNAi to develop disease resistance. Stable expression of PG-RNAi construct in rice showed efficient sil...Continue Reading

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Citations

Dec 8, 2019·Plant Biotechnology Journal·Kutubuddin A MollaKarabi Datta
Mar 2, 2021·Molecular Plant-microbe Interactions : MPMI·Zhengkun ZhangWende Liu
Oct 16, 2021·Molecular Plant-microbe Interactions : MPMI

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Datasets Mentioned

BETA
PRJNA523516

Methods Mentioned

BETA
transgenic
RNAseq
electrophoresis
PCR
Fluorescence
GTPase
transgenics

Software Mentioned

Trimmomatic
Blast2GO
TGICL ( TIGR Gene Indices
BLAST
BlastX
EvidentialGene assembler
Leica IM
BlastP

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