PMID: 6982071Aug 12, 1982Paper

Peculiar behaviour of rabbit thymocytes in interaction with liposomes of different compositions shown by fluorescence polarization studies, lipid analysis, and uptake of vesicle-entrapped carboxyfluorescein

Biochimica Et Biophysica Acta
R C Roozemond, D C Urli

Abstract

In order to obtain more information on membrane phenomena occurring at the cell surface of rabbit thymocytes we have performed experiments aimed at altering the lipid composition of the plasma membrane. Thymocytes were incubated at 37 degree C with phospholipid vesicles of different compositions. Vesicle-cell interaction was followed by measuring the degree of fluorescence polarization and the uptake of vesicle-entrapped carboxyfluorescein. Neutral and negatively charged liposomes prepared from egg phosphatidylcholine are currently used in investigations of vesicle-cell interaction. In this report we show that these liposomes do not interact with rabbit thymocytes as is evident from unaltered lipid fluidity measured in whole cells and in isolated plasma membranes. This was confirmed by experiments with vesicle-entrapped carboxyfluorescein showing hardly any uptake of the fluorophor from neutral and negatively charged egg phosphatidylcholine liposomes. Using both techniques substantial interaction was found with positively charged egg phosphatidylcholine liposomes and with liposomes prepared from soybean lecithin which is composed of a variety of phospholipids. The results of these experiments were supported by lipid analysis of...Continue Reading

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Citations

Apr 1, 1986·The Journal of Cell Biology·V S GoldmacherB C Nelson
Jan 1, 1987·International Journal of Radiation Applications and Instrumentation. Part B, Nuclear Medicine and Biology·A I Kassis, R A Taube
Apr 25, 1984·Biochimica Et Biophysica Acta·R A SchwendenerY E Rahman
Apr 1, 1986·Medicinal Research Reviews·S M SullivanL Huang
Sep 11, 1986·Biochimica Et Biophysica Acta·A R Nicholas, M N Jones

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