PEDF improves cardiac function in rats with acute myocardial infarction via inhibiting vascular permeability and cardiomyocyte apoptosis

International Journal of Molecular Sciences
Hao ZhangHongyan Dong

Abstract

Pigment epithelium-derived factor (PEDF) is a pleiotropic gene with anti-inflammatory, antioxidant and anti-angiogenic properties. However, recent reports about the effects of PEDF on cardiomyocytes are controversial, and it is not known whether and how PEDF acts to inhibit hypoxic or ischemic endothelial injury in the heart. In the present study, adult Sprague-Dawley rat models of acute myocardial infarction (AMI) were surgically established. PEDF-small interfering RNA (siRNA)-lentivirus (PEDF-RNAi-LV) or PEDF-LV was delivered into the myocardium along the infarct border to knockdown or overexpress PEDF, respectively. Vascular permeability, cardiomyocyte apoptosis, myocardial infarct size and animal cardiac function were analyzed. We also evaluated PEDF's effect on the suppression of the endothelial permeability and cardiomyocyte apoptosis under hypoxia in vitro. The results indicated that PEDF significantly suppressed the vascular permeability and inhibited hypoxia-induced endothelial permeability through PPARγ-dependent tight junction (TJ) production. PEDF protected cardiomyocytes against ischemia or hypoxia-induced cell apoptosis both in vivo and in vitro via preventing the activation of caspase-3. We also found that PEDF s...Continue Reading

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Citations

May 18, 2016·International Journal of Molecular Sciences·Shengcheng YaoKaijin Guo
Dec 16, 2016·International Journal of Molecular Sciences·Zhongxin ZhouZhongming Zhang
Nov 1, 2015·Apoptosis : an International Journal on Programmed Cell Death·Hao ZhangHongyan Dong
May 17, 2018·International Journal of Molecular Medicine·Hong-Xu JinSu-Nuan Zhao
Mar 8, 2018·International Journal of Molecular Medicine·Yufeng LiZhongming Zhang

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Methods Mentioned

BETA
co-immunoprecipitation
co-IP
transfection
sedation
Assay

Software Mentioned

Pro Plus
SPSS13
Image

Related Concepts

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Apoptosis

Apoptosis is a specific process that leads to programmed cell death through the activation of an evolutionary conserved intracellular pathway leading to pathognomic cellular changes distinct from cellular necrosis