Pediatric acute lymphoblastic leukemia-Conquering the CNS across the choroid plexus

Leukemia Research
Martin MärzMichael Karremann

Abstract

Despite the high prevalence of central nervous system (CNS) involvement in relapsing pediatric acute lymphoblastic leukemia (ALL), our understanding of CNS invasion is still vague. As lymphoblasts have to overcome the physiological blood-CNS barriers to enter the CNS, we investigated the cellular interactions of lymphoblasts with the choroid plexus (CP) epithelium of the blood-cerebrospinal fluid barrier (BCSFB). Both a precurser B cell ALL (pB-ALL) cell line (SD-1) and a T cell ALL (T-ALL) cell line (P12-Ishikawa) were able to actively cross the CP epithelium in a human in vitro model. We could illustrate a transcellular and (supposedly) paracellular transmigration by 3-dimensional immunofluorescence microscopy as well as electron microscopy. Chemotactic stimulation with CXCL12 during this process led to a significantly increased transmigration and blocking CXCL12/CXCR4-signaling by the CXCR4-inhibitor AMD3100 inhibited this effect. However, CXCR4 expression in primary ALL samples did not correlate to CNS disease, indicating that CXCR4-driven CNS invasion across the BCSFB might be a general property of pediatric ALL. Notably, we present a unique in vitro BCSFB model suitable to study CNS invasion of lymphoblasts in a human set...Continue Reading

References

Jan 15, 2003·Journal of Clinical Oncology : Official Journal of the American Society of Clinical Oncology·Britta BürgerMartin Schrappe
Mar 7, 2006·Leukemia Research·Arik EisenkraftGideon Paret
Mar 1, 2008·The Lancet Oncology·Ching-Hon Pui, Scott C Howard
Jun 26, 2009·The New England Journal of Medicine·Ching-Hon PuiMary V Relling
Jan 29, 2011·Leukemia Research·Stephen M AkersLaura F Gibson
Aug 21, 2012·Cell Adhesion & Migration·Rick B MeekerLola C Hudson
Aug 29, 2012·Trends in Immunology·Britta Engelhardt, Richard M Ransohoff
Jan 12, 2013·Fluids and Barriers of the CNS·Tobias TenenbaumHorst Schroten
Feb 26, 2013·Nature Reviews. Immunology·Ravid ShechterMichal Schwartz
Aug 6, 2015·Clinical & Translational Oncology : Official Publication of the Federation of Spanish Oncology Societies and of the National Cancer Institute of Mexico·N HeidariA Ahmadzadeh
Aug 26, 2015·Journal of Clinical Oncology : Official Journal of the American Society of Clinical Oncology·Ching-Hon PuiCharles G Mullighan
Jan 13, 2016·Journal of Clinical Oncology : Official Journal of the American Society of Clinical Oncology·Ajay VoraMaria Grazia Valsecchi
Mar 5, 2016·Journal of Leukocyte Biology·Tanja Rezzonico JostFabio Grassi
Jul 23, 2016·Pediatric Blood & Cancer·Mette LevinsenUNKNOWN Nordic Society of Pediatric Hematology and Oncology (NOPHO)
Oct 22, 2016·British Journal of Haematology·Liron Frishman-Levy, Shai Izraeli
Nov 11, 2016·Leukemia & Lymphoma·Jordan A Naumann, Peter M Gordon
May 12, 2017·Frontiers in Pediatrics·Nathan P Gossai, Peter M Gordon
Jan 26, 2018·Acta Neuropathologica·Jean-François Ghersi-EgeaBritta Engelhardt
Feb 13, 2018·Stem Cell Research & Therapy·Rita de Cassia Carvalho MeloSara Teresinha Olalla Saad

❮ Previous
Next ❯

Citations

Apr 19, 2019·Laboratory Investigation; a Journal of Technical Methods and Pathology·Alexa N LauerChristian Schwerk
Aug 6, 2020·International Journal of Molecular Sciences·Ulrike ErbMichael Karremann
Nov 2, 2019·Journal of Neuroscience Methods·Ulrike ErbMichael Karremann
May 7, 2021·Nature Reviews. Cancer·Andrew E WhiteleyDorothy A Sipkins
Jun 24, 2019·Pathology, Research and Practice·Xi ChenYinghua Li
Jan 4, 2022·Cell Adhesion & Migration·Juan D Diaz-ValenciaGenaro Patiño-López
Dec 23, 2021·Neuropathology and Applied Neurobiology·Oliver CousinsFederico Roncaroli

❮ Previous
Next ❯

Related Concepts

Related Feeds

Birth Defects

Birth defects encompass structural and functional alterations that occur during embryonic or fetal development and are present since birth. The cause may be genetic, environmental or unknown and can result in physical and/or mental impairment. Here is the latest research on birth defects.

Blood Brain Barrier

The blood brain barrier is a border that separates blood from cerebrospinal fluid. Discover the latest search on this highly selective semipermeable membrane here.

Blood Brain Barrier Chips

The blood brain barrier (BBB) is comprised of endothelial cells that regulate the influx and outflux of plasma concentrations. Lab-on-a-chip devices allow scientists to model diseases and mechanisms such as the passage of therapeutic antibodies across the BBB. Discover the latest research on BBB chips here.

CSF & Lymphatic System

This feed focuses on Cerebral Spinal Fluid (CSF) and the lymphatic system. Discover the latest papers using imaging techniques to track CSF outflow into the lymphatic system in animal models.

Blood And Marrow Transplantation

The use of hematopoietic stem cell transplantation or blood and marrow transplantation (bmt) is on the increase worldwide. BMT is used to replace damaged or destroyed bone marrow with healthy bone marrow stem cells. Here is the latest research on bone and marrow transplantation.