PMID: 8698665Jun 1, 1996

Pediocin 5 production and plasmid stability during continuous free and immobilized cell cultures of Pediococcus acidilactici UL5

The Journal of Applied Bacteriology
J HuangR E Simard

Abstract

Continuous production of pediocin 5 from Pediococcus acidilactici UL5 was investigated in MRS medium at different pH and dilution rates during continuous free cell (FC) and immobilized cell (IC) fermentations. Pediocin 5 activity from FC operated at a dilution rate of 0.31 h-1 largely increased from 128 to 2048 AU mL-1 as pH decreased from 7.0 to 5.0. Pediocin 5 activity in IC at a dilution rate of 0.93 h-1 was much less affected by pH, varying from 256 AU mL-1 at pH 7.0 to 512 AU mL-1 at pH 5.0. At the optimum pH 5.0, the dilution rate greatly influenced pediocin 5 activity both in FC and IC. Pediocin 5 production during continuous FC culture decreased with time for all dilution rates tested except 0.31 h-1 and average activity over 144 h cultures reached a maximal value of 4915 AU mL-1 at a dilution rate of 0.26 h-1. For IC, pediocin 5 production was stable with time and increased with the dilution rate from 256 to 1024 AU mL-1 in the range of 0.47-2.28 h-1. Three Listeria strains were tested for their ability to screen low bacteriocin-producing variants (Bac+v) of Bac+ cells in FC and IC cultures by using a modified deferred antagonism method. Ten to 28% of Bac+v cells appeared after 144 h of FC cultures at dilution rates in...Continue Reading

References

Jun 1, 1990·Journal of Biotechnology·P K Kumar, K Schügerl
Dec 1, 1991·Applied and Environmental Microbiology·H DabaC Lacroix
Jun 1, 1989·Scandinavian Journal of Clinical and Laboratory Investigation·B GougetA Truchaud
Jan 1, 1994·Critical Reviews in Biotechnology·S Norton, J C Vuillemard
Sep 1, 1993·FEMS Microbiology Reviews·T R Klaenhammer

Citations

Apr 2, 2009·Journal of Industrial Microbiology & Biotechnology·O SimşekMustafa Akçelik
Oct 29, 2000·Journal of Applied Microbiology·A G ScannellE K Arendt
Jan 10, 2009·Microbial Cell Factories·Maria Papagianni, Sofia Anastasiadou
Dec 22, 2011·International Journal of Microbiology·Christopher T Lohans, John C Vederas
Jan 15, 2014·Fish Physiology and Biochemistry·Bidhan C DeA P Sharma
May 19, 2009·International Journal of Food Microbiology·Svetoslav D Todorov, L M Dicks
Jun 19, 2008·Journal of Bioscience and Bioengineering·Karim NaghmouchiC Lacroix
Jul 7, 2007·Food Microbiology·Karim NaghmouchiIsmaïl Fliss

Related Concepts

Lantibiotics
Hydrogen-Ion Concentration
Pediococcus
Episomes
Biomass

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Synthetic Genetic Array Analysis

Synthetic genetic arrays allow the systematic examination of genetic interactions. Here is the latest research focusing on synthetic genetic arrays and their analyses.

Neural Activity: Imaging

Imaging of neural activity in vivo has developed rapidly recently with the advancement of fluorescence microscopy, including new applications using miniaturized microscopes (miniscopes). This feed follows the progress in this growing field.

Computational Methods for Protein Structures

Computational methods employing machine learning algorithms are powerful tools that can be used to predict the effect of mutations on protein structure. This is important in neurodegenerative disorders, where some mutations can cause the formation of toxic protein aggregations. This feed follows the latests insights into the relationships between mutation and protein structure leading to better understanding of disease.

Congenital Hyperinsulinism

Congenital hyperinsulinism is caused by genetic mutations resulting in excess insulin secretion from beta cells of the pancreas. Here is the latest research.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Epigenetic Memory

Epigenetic memory refers to the heritable genetic changes that are not explained by the DNA sequence. Find the latest research on epigenetic memory here.

Cell Atlas of the Human Eye

Constructing a cell atlas of the human eye will require transcriptomic and histologic analysis over the lifespan. This understanding will aid in the study of development and disease. Find the latest research pertaining to the Cell Atlas of the Human Eye here.

Femoral Neoplasms

Femoral Neoplasms are bone tumors that arise in the femur. Discover the latest research on femoral neoplasms here.