PMID: 7539419Jun 1, 1995Paper

Pediococcus acidilactici ldhD gene: cloning, nucleotide sequence, and transcriptional analysis

Journal of Bacteriology
D GarmynJ Delcour

Abstract

The gene encoding D-lactate dehydrogenase was isolated on a 2.9-kb insert from a library of Pediococcus acidilactici DNA by complementation for growth under anaerobiosis of an Escherichia coli lactate dehydrogenase and pyruvate-formate lyase double mutant. The nucleotide sequence of ldhD encodes a protein of 331 amino acids (predicted molecular mass of 37,210 Da) which shows similarity to the family of D-2-hydroxyacid dehydrogenases. The enzyme encoded by the cloned fragment is equally active on pyruvate and hydroxypyruvate, indicating that the enzyme has both D-lactate and D-glycerate dehydrogenase activities. Three other open reading frames were found in the 2.9-kb insert, one of which (rpsB) is highly similar to bacterial genes coding for ribosomal protein S2. Northern (RNA) blotting analyses indicated the presence of a 2-kb dicistronic transcript of ldhD (a metabolic gene) and rpsB (a putative ribosomal protein gene) together with a 1-kb monocistronic rpsB mRNA. These transcripts are abundant in the early phase of exponential growth but steadily fade away to disappear in the stationary phase. Primer extension analysis identified two distinct promoters driving either cotranscription of ldhD and rpsB or transcription of rpsB ...Continue Reading

References

Dec 1, 1977·Proceedings of the National Academy of Sciences of the United States of America·F SangerA R Coulson
Sep 15, 1992·European Journal of Biochemistry·S KochharD J Nicholls
Jun 1, 1992·European Journal of Biochemistry·V S LamzinK S Wilson
May 29, 1991·Philosophical Transactions of the Royal Society of London. Series B, Biological Sciences·C R DunnJ J Holbrook
Nov 1, 1990·Proceedings of the National Academy of Sciences of the United States of America·O ResnekovA von Gabain
Jan 1, 1989·Journal of Bacteriology·F Mat-JanD P Clark
Dec 29, 1989·Biochemical and Biophysical Research Communications·G A Grant
Jul 19, 1974·Nature·M G RossmannK W Olsen
Mar 1, 1980·Microbiological Reviews·E I Garvie
Jan 1, 1994·DNA Research : an International Journal for Rapid Publication of Reports on Genes and Genomes·N OgasawaraH Yoshikawa
May 1, 1994·Molecular Microbiology·L J RasmussenA Løbner-Olesen
Feb 25, 1994·Journal of Molecular Biology·V S LamzinK S Wilson
Mar 4, 1994·Journal of Molecular Biology·J D GoldbergP Brick
Feb 1, 1994·Journal of Bacteriology·T FerainJ Delcour
Sep 1, 1992·Applied and Environmental Microbiology·A FitzsimonsM O'connell

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Citations

Mar 11, 2000·European Journal of Biochemistry·S KochharJ E Germond
Jan 24, 2006·Journal of Biochemistry·Yoshirou IshikuraHayao Taguchi
Oct 1, 1996·Antonie van Leeuwenhoek·B E DavidsonA J Hillier

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