Apr 10, 2020

Refining the timing of recombination rate plasticity in response to temperature in Drosophila pseudoobscura

BioRxiv : the Preprint Server for Biology
U. H. AltindagLaurie S Stevison


Meiotic recombination rates vary in response to environmental factors like temperature. Variation in recombination generates an additional source for genetic variation while errors in this pathway can lead to cancer and chromosome number anomalies. Estimating duration and sensitivity of a meiotic response to environmental perturbation requires an understanding of molecular events and well-designed experimental approaches. An earlier study suggested that the peak (most sensitive) timing of plasticity in Drosophila melanogaster occurred during the pre-meiotic interphase where DNA replication takes place in S-phase. Recently, heat stress has been shown to lead to plasticity in recombination rates in D. pseudoobscura. Here, a combination of molecular genotyping and a series of mutant screens were used to determine peak plasticity timing in this species. Mutant flies were reared in either control or stress temperatures in a traditional cross design. Using mixed model analysis, the odds of crossover formation was 1.53X higher during days 7-9 (p<0.0017) and 1.41X higher on day 9 (p<0.022) in high temperature as compared to control crosses, suggesting the time period as the timing of peak plasticity. Time of peak plasticity at day 9 in...Continue Reading

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Mentioned in this Paper

Genome-Wide Association Study
Methyl coenzyme M
Transcription, Genetic
Gene Expression
Cell Type
Regulatory Sequences, Nucleic Acid
Enhancer of transcription
WA01 Cell Line

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