PEF based hurdle strategy to control Pichia fermentans, Listeria innocua and Escherichia coli k12 in orange juice

International Journal of Food Microbiology
C McNameeA G M Scannell

Abstract

The combination of pulsed electric fields (PEF) and bacteriocins in a hurdle approach has been reported to enhance microbial inactivation. This study investigates the preservation of orange juice using PEF in combination with nisin (2.5 ppm), natamycin (10 ppm), benzoic acid (BA; 100 ppm), or lactic acid, (LA; 500 ppm). Pichia fermentans, a spoilage yeast frequently isolated from orange juice, Escherichia coli k12 or Listeria innocua were inoculated into sterile orange juice (OJ) with, and without, added preservatives. The antimicrobial activity over time was evaluated relative to an untreated control. The effect of PEF treatment (40 kV/cm, 100 micros; max temperature 56 degrees C) was assessed on its own, and in combination with each antimicrobial. The acidic environment of OJ inactivated E. coli k12 (1.5log reduction) and L. innocua (0.7log reduction) slightly but had no effect on P. fermentans. PEF caused a significant decrease (P<0.05) in the viability of P. fermentans, L. innocua and E. coli k12 achieving reductions of 4.8, 3.7 and 6.3log respectively. Nisin combined with PEF inactivated L. innocua and E. coli k12 in a synergistic manner resulting in a total reduction to 5.6 and 7.9log respectively. A similar synergy was s...Continue Reading

References

Jun 3, 1975·The Journal of Membrane Biology·H G Coster, U Simmermann
Aug 1, 1991·Biophysical Journal·T Y Tsong
Mar 1, 1996·Letters in Applied Microbiology·F SchvedB J Juven
Jan 1, 1997·Critical Reviews in Microbiology·M E Parish
Oct 8, 1999·Emerging Infectious Diseases·P S MeadR V Tauxe
May 2, 2000·Applied and Environmental Microbiology·H L AlakomiI M Helander
May 3, 2000·International Journal of Food Microbiology·L Leistner
Mar 28, 2002·Applied and Environmental Microbiology·Covadonga R AriasMickey E Parish
Apr 5, 2002·Microbes and Infection·Beatrice H Lado, Ahmed E Yousef
May 27, 2005·Journal of Applied Microbiology·P Mañas, R Pagán
Oct 3, 2006·International Journal of Food Microbiology·Megha Gandhi, Michael L Chikindas
Jul 7, 2007·International Journal of Food Microbiology·Antonio GálvezNabil Ben Omar
Jan 1, 2008·The Journal of Biological Chemistry·Yvonne M te WelscherEefjan Breukink

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Citations

May 1, 2013·Critical Reviews in Food Science and Nutrition·Paulraj KanmaniVenkatesan Arul
Sep 2, 2011·International Journal of Food Microbiology·G SaldañaI Alvarez
Mar 4, 2014·Critical Reviews in Food Science and Nutrition·G SaldañaJ Raso
Apr 26, 2018·Critical Reviews in Food Science and Nutrition·Lakhsmi Sammugam, Visweswara Rao Pasupuleti
Jan 1, 2018·Comprehensive Reviews in Food Science and Food Safety·Antonio BevilacquaMaria Rosaria Corbo
Sep 1, 2013·Comprehensive Reviews in Food Science and Food Safety·Roman BuckowStefan Toepfl
Mar 1, 2018·Comprehensive Reviews in Food Science and Food Safety·Ume RoobabAlaa El-Din Bekhit
Dec 9, 2020·Plant Disease·Zachary D ZalewskiPatricia S McManus

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