PEG 6000-Stimulated Drought Stress Improves the Attributes of In Vitro Growth, Steviol Glycosides Production, and Antioxidant Activities in Stevia rebaudiana Bertoni

Plants
Muhammad Arslan AhmadYuesuo Yang

Abstract

This study addresses the favourable effects of drought stress imposed by polyethylene glycol (PEG) 6000 on the micropropagated shoots of Stevia rebaudiana. Various concentrations, i.e., 0, 0.5, 1, 2, and 4% of PEG 6000 were applied to the nodal shoot explants for four weeks, and the influence produced on shoots growth parameters, bioactive steviol glycosides (rebaudioside A and stevioside), and nonenzymatic antioxidant activities (total phenolic content (TPC), total flavonoid content (TFC), total antioxidant capacity (TAC), total reducing power (TRP) and 1,1-diphenyl-2-picrylhydrazyl(DPPH)-free radical scavenging activity (FRSA)) was elucidated. The significantly highest yield (92.4% direct shoot organogenesis) and secondary metabolites (2.94% Reb A, 2.52% ST, 95.3% DPPH-FRSA, 15.0% TPC, 13.0 µg/mg TFC, 22.3 µg/mg TAC, and 19.8 µg/mg TRP) production in response to abiotic stress elicitors was obtained in Murashige and Skoog (MS) medium treatment provided with 4% of PEG 6000. The overall trend was significant enhancement of growth dynamics and pharmaceutical compounds from control to 4% of PEG 6000 concentration as a defensive response against reactive oxygen species (ROS) produced in excess by water deficit. This is a very prom...Continue Reading

References

Nov 13, 2012·Journal of Diabetes and Its Complications·Naveen ShivannaVijay K Kaul
Mar 8, 2014·Applied Biochemistry and Biotechnology·Shokoofeh Hajihashemi, Ali Akbar Ehsanpour
Mar 9, 2018·Physiology and Molecular Biology of Plants : an International Journal of Functional Plant Biology·Shokoofeh HajihashemiIvica Djalovic
May 8, 2018·Planta·Claudia A Espinosa-LealSilverio García-Lara
Apr 11, 2019·Antioxidants·Miriam LaxaKarl-Josef Dietz
Mar 21, 2020·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Muhammad Arslan AhmadYuesuo Yang

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