PEGylated human serum albumin (HSA) nanoparticles: preparation, characterization and quantification of the PEGylation extent

Nanotechnology
E FahrländerK Langer

Abstract

Modification with poly(ethylene glycol) (PEG) is a widely used method for the prolongation of plasma half-life of colloidal carrier systems such as nanoparticles prepared from human serum albumin (HSA). However, the quantification of the PEGylation extent is still challenging. Moreover, the influence of different PEG derivatives, which are commonly used for nanoparticle conjugation, has not been investigated so far. The objective of the present study is to develop a method for the quantification of PEG and to monitor the influence of diverse PEG reagents on the amount of PEG linked to the surface of HSA nanoparticles. A size exclusion chromatography method with refractive index detection was established which enabled the quantification of unreacted PEG in the supernatant. The achieved results were confirmed using a fluorescent PEG derivative, which was detected by photometry and fluorimetry. Additionally, PEGylated HSA nanoparticles were enzymatically digested and the linked amount of fluorescently active PEG was directly determined. All the analytical methods confirmed that under optimized PEGylation conditions a PEGylation efficiency of up to 0.5 mg PEG per mg nanoparticle could be achieved. Model calculations made a 'brush' ...Continue Reading

References

Apr 1, 1995·Journal of Pharmaceutical Sciences·D BazileM Veillard
Apr 1, 1997·Biomaterials·W LinL Illum
Dec 22, 1999·International Journal of Pharmaceutics·C WeberK Langer
Jan 4, 2001·International Journal of Pharmaceutics·C WeberK Langer
Jun 8, 2002·Advanced Drug Delivery Reviews·Francesco M Veronese, J Milton Harris
Jun 8, 2002·Advanced Drug Delivery Reviews·M J RobertsJ M Harris
Mar 4, 2003·Nature Reviews. Drug Discovery·J Milton Harris, Robert B Chess
Apr 25, 2003·International Journal of Pharmaceutics·K LangerD Schubert
Sep 23, 2003·Advanced Drug Delivery Reviews·Paolo Caliceti, Francesco M Veronese
Sep 10, 2005·European Journal of Pharmaceutical Sciences : Official Journal of the European Federation for Pharmaceutical Sciences·Chao FangHong-Zhuan Chen
Oct 26, 2005·Drug Discovery Today·Francesco M Veronese, Gianfranco Pasut
Nov 24, 2005·International Journal of Pharmaceutics·Donald E Owens, Nicholas A Peppas
Jun 28, 2008·Journal of Controlled Release : Official Journal of the Controlled Release Society·Felix Kratz
Jul 15, 2009·Biochimica Et Biophysica Acta·Shyh-Dar Li, Leaf Huang
Aug 13, 2009·Advanced Drug Delivery Reviews·Gianfranco Pasut, Francesco M Veronese
Sep 17, 2009·The Journal of Physical Chemistry. B·Hwankyu LeeRichard W Pastor
Mar 27, 2010·Journal of Controlled Release : Official Journal of the Controlled Release Society·Shyh-Dar Li, Leaf Huang
Jul 2, 2011·Nanomedicine·Jesse V JokerstSanjiv S Gambhir
Nov 19, 2011·Journal of Controlled Release : Official Journal of the Controlled Release Society·Gianfranco Pasut, Francesco M Veronese
Apr 26, 2012·Biotechnology Journal·Noriko Yoshimoto, Shuichi Yamamoto
Oct 10, 2013·Journal of Separation Science·Xiaojiao ShangRaja Ghosh
Dec 25, 2013·European Journal of Pharmaceutics and Biopharmaceutics : Official Journal of Arbeitsgemeinschaft Für Pharmazeutische Verfahrenstechnik E.V·Waralee WatcharinJörg Kreuter
Mar 19, 2014·Journal of Controlled Release : Official Journal of the Controlled Release Society·Felix Kratz

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Citations

Oct 30, 2016·Nanomedicine : Nanotechnology, Biology, and Medicine·Eduardo FuentesCristian Vilos
Apr 3, 2018·Chemical & Pharmaceutical Bulletin·Kotaro KimuraMasaki Otagiri
Nov 5, 2016·IEEE Transactions on Ultrasonics, Ferroelectrics, and Frequency Control·Helen MulvanaEleanor Stride
Jan 10, 2020·Archives of Pharmacal Research·Shrawani Lamichhane, Sangkil Lee
Apr 1, 2021·Molecular Pharmaceutics·Alessandra SpadaAfsaneh Lavasanifar

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