PEGylated lipids impede the lateral diffusion of adsorbed proteins at the surface of (magneto)liposomes

Colloids and Surfaces. B, Biointerfaces
N NuyttenM de Cuyper

Abstract

Protein binding to nanoparticles is a crucial issue in biomedicine, as it triggers their clearance from the bloodstream after intravenous injection. Many techniques are available for measuring strong protein binding interactions, but weak dynamic interactions are more difficult to assess. To tackle the latter problem, in the present work, cytochrome c was chosen as a representative model of a water-soluble protein and the adsorbing particulates were either small unilamellar phospholipid vesicles or 14 nm diameter solid superparamagnetic iron oxide cores onto which a phospholipid bilayer was strongly chemisorbed (so-called magnetoliposomes). Incorporation of cytochrome c oxidase into the phospholipid bilayer allowed the association of cytochrome c with the surface of the particles to be measured with high sensitivity by VIS-spectrophotometry. The impact of enzyme density as well as some of the physical features of the PEG corona (degree of PEGylation and PEG chain length) adjacent to the surface of the lipid structures on the overall kinetics was also investigated.

References

Jul 23, 1991·Biochemistry·A MugaW K Surewicz
Jun 1, 2005·Chemistry and Physics of Lipids·Olga GarbuzenkoAba Priev
Jan 13, 2006·Chemistry and Physics of Lipids·Sophia PiperoudiSophia G Antimisiaris
May 3, 2006·Biomaterials·Arnaud VonarbourgJean-Pierre Benoit
Oct 15, 2009·The European Physical Journal. E, Soft Matter·G Fragneto
May 21, 2008·Journal of Physics. Condensed Matter : an Institute of Physics Journal·J CocquytM De Cuyper

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Citations

Jan 18, 2013·Therapeutic Delivery·Aristides BakandritsosDimitrios G Fatouros
Feb 21, 2016·Colloids and Surfaces. B, Biointerfaces·Fikret AydinMeenakshi Dutt
Jun 28, 2011·Nanomedicine : Nanotechnology, Biology, and Medicine·Athanasios SkourasSophia G Antimisiaris
May 28, 2020·International Journal of Molecular Sciences·Beatriz D CardosoPaulo J G Coutinho
Nov 27, 2018·Royal Society Open Science·Beatriz D CardosoPaulo J G Coutinho

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