PEITC inhibits human brain glioblastoma GBM 8401 cell migration and invasion through the inhibition of uPA, Rho A, and Ras with inhibition of MMP-2, -7 and -9 gene expression

Oncology Reports
Yu-Cheng ChouJing-Gung Chung

Abstract

Glioblastoma is the most aggressive primary brain malignancy, and the efficacy of multimodality treatments remains unsatisfactory. Phenethyl isothiocyanate (PEITC), one member of the isothiocyanate family, was found to inhibit the migration and invasion of many types of human cancer cells. In our previous study, PEITC induced the apoptosis of human brain glioblastoma GBM 8401 cells through the extrinsic and intrinsic signaling pathways. In the present study, we first investigated the effects of PEITC on the migration and invasion of GBM 8401 cells. PEITC decreased the migration of GBM 8401 cells in a dose-dependent manner as determined from scratch wound healing and Transwell migration assays. The percentage of inhibition ranged from 46.89 to 15.75%, and from 27.80 to 7.31% after a 48-h treatment of PEITC as determined from the Transwell migration assay and invasion assay, respectively. The western blot analysis indicated that PEITC decreased the levels of proteins associated with migration and invasion, Ras, uPA, RhoA, GRB2, p-p38, p-JNK, p-ERK, p65, SOS1, MMP-2, MMP-9 and MMP-13, in a dose-dependent manner. Real-time PCR analyses revealed that PEITC reduced the mRNA levels of MMP-2, MMP-7, MMP-9 and RhoA in a dose- and time-d...Continue Reading

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Citations

May 18, 2019·The American Journal of Chinese Medicine·Fei-Ting HsuJing-Gung Chung
Jun 29, 2017·International Journal of Oncology·Sanghun LeeHye Jin Jung
Sep 5, 2019·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Emma J van BodegravenElly M Hol
Jul 7, 2018·Journal of Food and Drug Analysis·Gow-Chin YenChia-Jui Weng
Mar 30, 2017·Tumour Biology : the Journal of the International Society for Oncodevelopmental Biology and Medicine·Jishu QuanMing Jin
Feb 1, 2017·Neuropathology : Official Journal of the Japanese Society of Neuropathology·Li XuGang Cui
Sep 12, 2018·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Yu-Cheng ChouJing-Gung Chung
Jan 13, 2021·Seminars in Cancer Biology·Madhumitha Kedhari SundaramArif Hussain
Dec 29, 2020·Frontiers in Oncology·Kristie LiuFrank J Attenello
Feb 23, 2021·Frontiers in Cellular Neuroscience·Matthew J RybinZane Zeier
Jun 12, 2018·Pharmacology & Therapeutics·Melina MitsiogianniMihalis I Panayiotidis

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