Penaeus monodon SERPIN, PmSERPIN6, is implicated in the shrimp innate immunity

Fish & Shellfish Immunology
Teerada HomvisesKunlaya Somboonwiwat

Abstract

Serine proteinase inhibitors (SERPINs or serpins) have been found in a diverse range of organisms. Herein, eight serpin genes, namely PmSERPIN1 - 8, were identified from the Penaeus monodon EST database (http://pmonodon.biotec.or.th/home.jsp). Among those, PmSERPIN6 was selected for further characterization. Tissue distribution analysis revealed that PmSERPIN6 transcripts were expressed in the lymphoid organ, hemocyte, heart and gill, but not in the hepatopancreas. Semi-quantitative RT-PCR analysis at 0-48 h after pathogen challenge demonstrated that the PmSERPIN6 gene transcript expression levels in hemocytes was slightly decreased after systemic white spot syndrome virus (WSSV) injection but remained unchanged upon Vibrio harveyi injection. Interestingly, immunocytochemistry using anti-PmSERPIN6 polyclonal antiserum showed an increase in the number of PmSERPIN6 producing hemocytes at 72 h after both WSSV and V. harveyi injections indicating that the expression of PmSERPIN6 responded to pathogen in the late phase of infection. Our results suggest a likely important function of PmSERPIN6 in the shrimp's defense against invading pathogens.

Citations

May 11, 2013·Developmental and Comparative Immunology·Natthiya WetsaphanKunlaya Somboonwiwat
Jan 15, 2014·Developmental and Comparative Immunology·Yan-Ran ZhaoJin-Xing Wang
Oct 13, 2012·Fish & Shellfish Immunology·Anchalee TassanakajonSureerat Tang
Sep 11, 2012·Fish & Shellfish Immunology·Piti AmparyupAnchalee Tassanakajon
Jun 29, 2012·Fish & Shellfish Immunology·Suphaphon SomnukVichien Rimphanitchayakit
Aug 15, 2015·Developmental and Comparative Immunology·Pakkakul SangsuriyaPiti Amparyup
Jun 25, 2016·Developmental and Comparative Immunology·Wipasiri SoonthornchaiPikul Jiravanichpaisal
Jan 10, 2018·Molecular Reproduction and Development·Charoonroj ChotwiwatthanakunRapeepun Vanichviriyakit
Dec 2, 2017·Archives of Insect Biochemistry and Physiology·Saima KausarChaoliang Liu
Mar 1, 2021·Ecotoxicology and Environmental Safety·Qun JiangXiaojun Zhang

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