Penaeus monodon thioredoxin restores the DNA binding activity of oxidized white spot syndrome virus IE1.

Antioxidants & Redox Signaling
Jiun-Yan HuangChu-Fang Lo

Abstract

In this study we identified viral gene targets of the important redox regulator thioredoxin (Trx), and explored in depth how Trx interacts with the immediate early gene #1 (IE1) of the white spot syndrome virus (WSSV). In a pull-down assay, we found that recombinant Trx bound to IE1 under oxidizing conditions, and a coimmunoprecipitation assay showed that Trx bound to WSSV IE1 when the transfected cells were subjected to oxidative stress. A pull-down assay with Trx mutants showed that no IE1 binding occurred when cysteine 62 was replaced by serine. Electrophoretic mobility shift assay (EMSA) showed that the DNA binding activity of WSSV IE1 was downregulated under oxidative conditions, and that Penaeus monodon Trx (PmTrx) restored the DNA binding activity of the inactivated, oxidized WSSV IE1. Another EMSA experiment showed that IE1's Cys-X-X-Cys motif and cysteine residue 55 were necessary for DNA binding. Measurement of the ratio of reduced glutathione to oxidized glutathione (GSH/GSSG) in WSSV-infected shrimp showed that oxidative stress was significantly increased at 48 h postinfection. The biological significance of Trx was also demonstrated in a double-strand RNA Trx knockdown experiment where suppression of shrimp Trx led...Continue Reading

References

Jan 1, 1985·Annual Review of Biochemistry·A Holmgren
Jan 1, 1996·Free Radical Biology & Medicine·K B Schwarz
Aug 26, 1998·Biochemical and Biophysical Research Communications·Y Huang, F E Domann
Jan 23, 1999·The Journal of Biological Chemistry·Y MakinoH Tanaka
Jun 8, 2000·FEMS Microbiology Reviews·R A FabianekL Thöny-Meyer
Feb 16, 2002·Methods : a Companion to Methods in Enzymology·K J Livak, T D Schmittgen
Jan 14, 2003·Annual Review of Biochemistry·Hiroshi KadokuraJon Beckwith
Jul 14, 2007·Developmental and Comparative Immunology·Jiann-Horng LeuChu-Fang Lo
May 8, 2008·Developmental and Comparative Immunology·Wei Yu ChenChu Fang Lo
May 16, 2008·Comparative Biochemistry and Physiology. Toxicology & Pharmacology : CBP·Emmanuel Aispuro-HernandezRogerio R Sotelo-Mundo
Oct 18, 2008·Molecular Aspects of Medicine·Alessandra FraternaleMauro Magnani
May 6, 2011·Archives of Virology·Fanyu LinFeng Yang
Jan 1, 1999·Alternatives to Laboratory Animals : ATLA·P P LeenaarsD E Stewart-Tull

❮ Previous
Next ❯

Citations

Jun 3, 2014·Developmental and Comparative Immunology·Ping-Han HuangLi-Kwan Chang
May 28, 2015·Journal of Invertebrate Pathology·Peng-fei LiuJie Huang

❮ Previous
Next ❯

Datasets Mentioned

BETA
AAL88872.1
AAL88907.1
AAL88976.1
AAL88981.1
AAL88994.1
AAL89002.1
AAL89122.1
AAL89227.1

Methods Mentioned

BETA
pull-down
pull down
electrophoresis
coimmunoprecipitation
electrophoretic mobility shift assay
PCR
protein assay
transfection

Software Mentioned

GraphPad Prism

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.