Pendant-bearing glucose-neopentyl glycol (P-GNG) amphiphiles for membrane protein manipulation: Importance of detergent pendant chain for protein stabilization.

Acta Biomaterialia
Hyoung Eun BaePil Seok Chae

Abstract

Glucoside detergents are successfully used for membrane protein crystallization mainly because of their ability to form small protein-detergent complexes. In a previous study, we introduced glucose neopentyl glycol (GNG) amphiphiles with a branched diglucoside structure that has facilitated high resolution crystallographic structure determination of several membrane proteins. Like other glucoside detergents, however, these GNGs were less successful than DDM in stabilizing membrane proteins, limiting their wide use in protein structural study. As a strategy to improve GNG efficacy for protein stabilization, we introduced two different alkyl chains (i.e., main and pendant chains) into the GNG scaffold while maintaining the branched diglucoside head group. Of these pendant-bearing GNGs (P-GNGs), three detergents (GNG-2,14, GNG-3,13 and GNG-3,14) were not only notably better than both DDM (a gold standard detergent) and the previously described GNGs at stabilizing all six membrane proteins tested here, but were also as efficient as DDM at membrane protein extraction. The results suggest that the C14 main chain of the P-GNGs is highly compatible with the hydrophobic widths of membrane proteins, while the C2/C3 pendant chain is effec...Continue Reading

References

Dec 24, 1996·Proceedings of the National Academy of Sciences of the United States of America·C TribetJ L Popot
Jul 20, 1999·Annual Review of Biophysics and Biomolecular Structure·S H White, W C Wimley
Jul 4, 2001·The Journal of Biological Chemistry·R M Garavito, S Ferguson-Miller
Aug 10, 2001·Current Opinion in Structural Biology·J U Bowie
Dec 26, 2001·Molecular Pharmacology·Gayathri SwaminathTae Weon Lee
Jan 14, 2003·Nature Biotechnology·Clare-Louise McGregorGilbert G Privé
Nov 6, 2004·Modern Pathology : an Official Journal of the United States and Canadian Academy of Pathology, Inc·Laura ChowJohn R Mackey
Jun 27, 2006·Nature Chemical Biology·Xiaojie YaoBrian Kobilka
Sep 16, 2006·Circulation Research·Matthew T DrakeRobert J Lefkowitz
Mar 16, 2007·Proceedings of the National Academy of Sciences of the United States of America·Matthias Quick, Jonathan A Javitch
May 8, 2007·Trends in Biochemical Sciences·Jean-Jacques LacapèreCatherine Etchebest
Jan 15, 2008·Proceedings of the National Academy of Sciences of the United States of America·Maria J Serrano-VegaChristopher G Tate
Jan 26, 2008·Protein Science : a Publication of the Protein Society·Simon NewsteadSo Iwata
Mar 13, 2008·Structure·Alexander I AlexandrovRaymond C Stevens
Oct 28, 2008·Methods : a Companion to Methods in Enzymology·Edmund R S KunjiPearl Akamine
Nov 22, 2008·Molecular Membrane Biology·Simon NewsteadSo Iwata
Jun 10, 2009·Optics Express·Markus A PlumBernhard Menges
Feb 16, 2010·Protein Expression and Purification·James LeungBernadette Byrne
Dec 15, 2010·The Journal of Biological Chemistry·Lan GuanSiva P Ankeshwarapu
Jan 5, 2011·Nature Reviews. Drug Discovery·Rosamaria Lappano, Marcello Maggiolini
Jan 14, 2011·Nature·Daniel M RosenbaumBrian K Kobilka
Jan 27, 2011·Journal of Cardiovascular Pharmacology·Rachel W S LiGeorge P H Leung
May 7, 2011·Annual Review of Biophysics·J-L PopotM Zoonens
Oct 1, 2011·Methods : a Companion to Methods in Enzymology·Qinghai ZhangWen-Xu Hong
Mar 23, 2012·Nature·Aashish ManglikSébastien Granier
Jul 28, 2012·Science·Juho KellosaloAdrian Goldman
Nov 21, 2012·Chemical Communications : Chem Comm·Pil Seok ChaeSamuel H Gellman
Mar 30, 2013·Science·Andrew QuigleyElisabeth P Carpenter
Jul 3, 2013·Nature Methods·Houchao TaoQinghai Zhang
Oct 15, 2013·Chemistry : a European Journal·Pil Seok ChaeSamuel H Gellman
Nov 22, 2013·Nature·Andrew C KruseBrian K Kobilka
Jan 7, 2014·Nature Communications·Abdul S EthayathullaLan Guan
Apr 16, 2014·Proceedings of the National Academy of Sciences of the United States of America·Anna FrickSusanna Törnroth-Horsefield
May 31, 2014·Science·Erkan Karakas, Hiro Furukawa
Oct 23, 2014·Nature·Veronica Kane DicksonStephen B Long
Apr 10, 2015·Nature·Candice E PaulsenDavid Julius

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