Penehyclidine hydrochloride post-conditioning reduces ischemia/reperfusion-induced cardiomyocyte apoptosis in rats

Experimental and Therapeutic Medicine
Hongbao TanJun Ma

Abstract

Ischemic heart disease is a major cause of mortality and disability worldwide. Timely reperfusion is currently the most effective method of treating ischemic heart disease; however, abrupt reperfusion may cause ischemia/reperfusion (I/R) injury. Apoptosis serves an important role in the progression of myocardial I/R injury and it has been demonstrated that the mitochondria are the center of regulation for apoptosis. Penehyclidine hydrochloride (PHC) is used during surgery and has recently been identified as a new type of anticholinergic drug. It has been demonstrated in vivo that pretreatment with PHC reduces myocardial apoptosis in rat hearts. The present study aimed to investigate the effects of PHC post-conditioning on myocardial cell apoptosis in a rat model of myocardial I/R and to determine whether the mitochondria-induced pathway was activated. Male Wistar rats were evenly and randomly categorized into 4 experimental groups as follows: i) Sham group; ii) I/R group; iii) PHC+sham group; and iv) PHC+I/R group. A PHC (1 mg/kg) post-conditioning approach (5 min before reperfusion) was used in addition to I/R in the PHC-treated groups. Following 3 h reperfusion, flow cytometry and terminal deoxynucleotidyl transferase dUTP ni...Continue Reading

References

Nov 1, 1996·Circulation Research·H Fliss, D Gattinger
Aug 10, 2000·Trends in Cell Biology·S Desagher, J C Martinou
Feb 14, 2004·Cardiovascular Research·Frank EeftingPieter A Doevendans
Jan 24, 2007·Physiological Reviews·Guido KroemerCatherine Brenner
Oct 14, 2009·Current Opinion in Cell Biology·Dirk Brenner, Tak W Mak
Mar 30, 2010·Molecular Aspects of Medicine·Varda Shoshan-BarmatzNir Arbel
Nov 9, 2010·Biochimica Et Biophysica Acta·Jennefer LindsayAndrew P Gilmore
Nov 10, 2010·The American Journal of Cardiology·Giancarlo MarenziPiergiuseppe Pelicci
May 3, 2011·Mitochondrion·Varda Shoshan-Barmatz, Danya Ben-Hail
Jun 28, 2011·Mitochondrion·Bernhard KadenbachSebastian Vogt
Apr 24, 2012·International Immunopharmacology·Hsiang-Ping LeeChih-Hsin Tang
May 19, 2012·Expert Opinion on Pharmacotherapy·Vikram SharmaDerek M Yellon
Sep 11, 2012·Trends in Cardiovascular Medicine·Stéphanie Barrère-LemaireChristophe Piot
Jun 26, 2014·Evidence-based Complementary and Alternative Medicine : ECAM·Anzhou XiaWeidong Zhou
Jan 17, 2015·Cellular Physiology and Biochemistry : International Journal of Experimental Cellular Physiology, Biochemistry, and Pharmacology·Xiaoming WangJun Ren
Apr 18, 2015·Biochemical and Biophysical Research Communications·Shu ZhangMing Cui
Jul 15, 2015·Experimental and Therapeutic Medicine·Xiao-Bo LiuCheng-Ri Cui
Dec 15, 2015·Cell Reports·Rahul PalchaudhuriPaul J Hergenrother
Apr 2, 2016·Current Drug Targets·Hong-Jie ChiJun Cai
Apr 16, 2016·Cellular Physiology and Biochemistry : International Journal of Experimental Cellular Physiology, Biochemistry, and Pharmacology·Tiebing ZhuJie Chao
Jun 28, 2016·Biochemical and Biophysical Research Communications·Yu QiaoHui Huang
Aug 31, 2016·Bioorganic & Medicinal Chemistry Letters·Yong Joo ParkKi Hyun Kim
Oct 30, 2016·European Journal of Pharmacology·Liang Xiao-FengWang Wen-Ya
Feb 12, 2017·Current Cardiology Reports·Amit V Patel, Sripal Bangalore
May 4, 2017·Journal of Translational Medicine·Elena Teringova, Petr Tousek

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Methods Mentioned

BETA
flow cytometry
Protein assay

Software Mentioned

CellQuest Pro
GeneTools
SPSS

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