Penetration enhancement of two topical 5-aminolaevulinic acid formulations for photodynamic therapy by erbium:YAG laser ablation of the stratum corneum: continuous versus fractional ablation

Experimental Dermatology
Bernadette ForsterTim Maisch

Abstract

5-Aminolaevulinic acid (ALA) is used in photodynamic therapy (PDT). Response rates of PDT vary widely, which may be because of the limited uptake of topically applied photosensitisers. We investigated skin penetration and fluorescence induction of protoporphyrin IX (PpIX) after applying either 20% ALA cream or 20% aminolaevulinic acid solution on laser-stripped stratum corneum (SC) in an ex vivo full-thickness porcine skin model. Both formulations are used in clinical practice. To enhance the skin penetration of ALA, we used two different 2940-nm erbium:yttrium-aluminium-garnet (Er:YAG) laser systems to partially ablate the SC: continuous and fractional ablation. Different fluences were applied ranging from 0.5 to 1.5 J/cm(2) (continuous ablation) and from 4 to 24 J/cm(2) (fractional ablation). Fluorescence microscopy was used for detecting PpIX-induced fluorescence. Compared to skin without laser pretreatment, mean fluorescence intensity (MFI) of PpIX was enhanced 13.8-fold after continuous ablation with 1.0 J/cm(2) and 7.3-fold after fractional ablation with 4 J/cm(2); each laser procedure was followed by 4-h incubation with lipophilic ALA cream. Optimal parameters for continuous ablation without damage to the epidermis were ...Continue Reading

References

Nov 1, 1991·The Journal of Investigative Dermatology·J S NelsonS L Jacques
Jan 1, 1987·The Journal of Investigative Dermatology·S L JacquesJ A Parrish
Jul 14, 2001·Journal of Controlled Release : Official Journal of the Controlled Release Society·W R LeeJ Y Fang
May 23, 2002·Photochemistry and Photobiology·Patricia G JohnsonAllan R Oseroff
Jul 13, 2002·Journal of Pharmaceutical Sciences·Woan-Ruoh LeeJia-You Fang
Feb 15, 2003·Journal of Controlled Release : Official Journal of the Controlled Release Society·Renata F V LopezRichard H Guy
Mar 3, 2004·Photochemical & Photobiological Sciences : Official Journal of the European Photochemistry Association and the European Society for Photobiology·Johanna T H M van den AkkerHenricus J C M Sterenborg
Aug 5, 2004·Archives of Dermatological Research·Heinrich DickelJochen Brasch
May 10, 2007·Photochemical & Photobiological Sciences : Official Journal of the European Photochemistry Association and the European Society for Photobiology·T MaischC Abels
May 9, 2008·Dermatologic Surgery : Official Publication for American Society for Dermatologic Surgery [et Al.]·Moshe LapidothLilian Mayumi Odo
Jun 17, 2008·Journal of Controlled Release : Official Journal of the Controlled Release Society·Ryan F DonnellyJohan Moan
Dec 17, 2008·Dermatology : International Journal for Clinical and Investigative Dermatology·M J P GerritsenP E J van Erp

❮ Previous
Next ❯

Citations

May 11, 2012·Der Hautarzt; Zeitschrift für Dermatologie, Venerologie, und verwandte Gebiete·P Babilas, M Landthaler
Jul 25, 2012·Lasers in Medical Science·Chun-Hsun HuangYu-Shien Ko
Jun 4, 2013·Dermatologic Surgery : Official Publication for American Society for Dermatologic Surgery [et Al.]·Jan LippertMarek Vlk
Feb 22, 2013·Expert Opinion on Drug Delivery·Sandra ScheiblhoferRichard Weiss
Mar 26, 2014·Lasers in Surgery and Medicine·Lindsay R SklarDavid M Ozog
Nov 28, 2016·Facial Plastic Surgery Clinics of North America·Jill S Waibel, Ashley Rudnick
Feb 10, 2017·Lasers in Surgery and Medicine·C S HaakM Haedersdal
Feb 17, 2018·Artificial Cells, Nanomedicine, and Biotechnology·Shahenda A RamezDalea M Aboel-Fadl
Dec 6, 2012·Lasers in Surgery and Medicine·Christina S HaakMerete Haedersdal
Mar 5, 2019·Dermatologic Surgery : Official Publication for American Society for Dermatologic Surgery [et Al.]·Hanan H SabryTaghreed A Abd El Azez
Apr 12, 2013·Journal of Biophotonics·Gustavo NicolodelliVanderlei Salvador Bagnato
May 14, 2014·Expert Review of Clinical Immunology·Jie ZhaoBo Huang
Apr 17, 2019·The Journal of Dermatological Treatment·Wei ZhaoHong-Qing Tian
May 19, 2016·Dermatologic Surgery : Official Publication for American Society for Dermatologic Surgery [et Al.]·Lisa Ann Zaleski-Larsen, Sabrina G Fabi
Feb 7, 2017·Current Dermatology Reports·Connor ThunshelleMichael R Hamblin
Feb 19, 2017·Lasers in Medical Science·Uffe Høgh OlesenMerete Haedersdal
Apr 30, 2020·Burns and Trauma·Kevin M KliftoC Scott Hultman
Mar 5, 2011·Experimental Dermatology·Olaf LademannStefan Koch
May 9, 2013·Journal der Deutschen Dermatologischen Gesellschaft = Journal of the German Society of Dermatology : JDDG·Rolf-Markus SzeimiesSigrid Karrer
Jan 4, 2012·Photodermatology, Photoimmunology & Photomedicine·Abdullah Al YousefPhilippe Saiag
Apr 28, 2016·Journal der Deutschen Dermatologischen Gesellschaft = Journal of the German Society of Dermatology : JDDG·Stephan Alexander BraunPeter Arne Gerber
Nov 30, 2012·Journal der Deutschen Dermatologischen Gesellschaft = Journal of the German Society of Dermatology : JDDG·Sigrid KarrerRolf-Markus Szeimies
Feb 22, 2012·The British Journal of Dermatology·K Togsverd-BoM Hædesdal
Apr 28, 2016·Journal der Deutschen Dermatologischen Gesellschaft = Journal of the German Society of Dermatology : JDDG·Stephan Alexander BraunPeter Arne Gerber
Nov 9, 2020·Lasers in Surgery and Medicine·Lewis McMillanKenneth Wood
Dec 11, 2020·Der Hautarzt; Zeitschrift für Dermatologie, Venerologie, und verwandte Gebiete·R-M Szeimies, S Karrer
Oct 20, 2019·Journal of Controlled Release : Official Journal of the Controlled Release Society·Sergio Del Río-SanchoMaría José Alonso
Apr 13, 2021·Photobiomodulation, Photomedicine, and Laser Surgery·Virginia GarofaloLuca Bianchi
Oct 31, 2020·Burns : Journal of the International Society for Burn Injuries·Andrea C Issler-FisherPeter K M Maitz

❮ Previous
Next ❯

Related Concepts

Related Feeds

Apoptosis

Apoptosis is a specific process that leads to programmed cell death through the activation of an evolutionary conserved intracellular pathway leading to pathognomic cellular changes distinct from cellular necrosis

Related Papers

Journal of Controlled Release : Official Journal of the Controlled Release Society
Woan-Ruoh LeeJia-You Fang
Journal of Controlled Release : Official Journal of the Controlled Release Society
Yogeshwar G BachhavYogeshvar N Kalia
Journal of Controlled Release : Official Journal of the Controlled Release Society
Woan-Ruoh LeeJia-You Fang
© 2021 Meta ULC. All rights reserved