Penetration of colicin M into cells of Escherichia coli.

Journal of Bacteriology
V BraunK Schaller

Abstract

A new class of colicin M-tolerant mutants of Escherichia coli K-12 was isolated. The mutants exhibited an unusually high tolerance in that they were unaffected by colicin titers of 10(6). The tolerance was confined to colicin M. It was mapped at a locus called tolM, which is close to rpsL. The following gene order was determined: aroE, tolM, rpsL, cysG. The tolerance could be caused by a defect in the uptake of colicin M or by a mutation at the site of action. Insensitive tonA and tonB mutants became sensitive to colicin M upon treatment by osmotic shock, whereas the tolM mutants remained insensitive. Trypsin rescue experiments showed that the tonB-dependent uptake of colicin M required energy like the other tonB-related transport processes. When bound to energy-depleted cells, colicin M prevented adsorption of phage T5. The receptor became accessible to the phage when the cells were energized, except in tonB mutants. These data suggest that the function controlled by the tonB gene is required for the translocation of colicin M from its initial binding site at the tonA-coded receptor protein to the target.

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Citations

Jan 1, 1991·Biology of Metals·V BraunK Hantke
Jul 1, 1991·FEMS Microbiology Reviews·R E Harkness, T Olschläger
Nov 28, 2012·Biochemical Society Transactions·Volkmar BraunSilke I Patzer
Aug 28, 2007·The Journal of Biological Chemistry·Daniel WalkerColin Kleanthous
Jul 22, 2008·The Journal of Biological Chemistry·Kornelius ZethVolkmar Braun
Nov 28, 2012·Biochemical Society Transactions·Thierry TouzéDominique Mengin-Lecreulx
May 1, 1991·Molecular Microbiology·T OlschlägerV Braun
Jan 1, 1984·Molecular & General Genetics : MGG·T OlschlägerV Braun
Sep 1, 1990·Molecular Microbiology·J Mende, V Braun
Jul 1, 1993·Molecular & General Genetics : MGG·H PilslV Braun
May 20, 2004·Journal of Bacteriology·Rajasekaran AnnamalaiPhillip E Klebba
Dec 15, 2010·Journal of Bacteriology·Stephanie Helbig, Volkmar Braun
Mar 10, 2007·Microbiology and Molecular Biology Reviews : MMBR·Eric CascalesDanièle Cavard
Dec 15, 2010·The Journal of Biological Chemistry·Stephanie HelbigVolkmar Braun
Jun 12, 1996·Molecular & General Genetics : MGG·P Gross, V Braun
Jan 1, 1985·Archives of Microbiology·R DreherB Wittmann-Liebold
Feb 18, 2010·The Journal of Biological Chemistry·Hélène BarreteauThierry Touzé
Dec 1, 1984·Archives of Microbiology·S Mollner, V Braun
May 29, 2007·Journal of Bacteriology·Wolfgang RabschPhillip E Klebba
Jun 17, 2008·Molecular Microbiology·Julia HullmannVolkmar Braun
Aug 18, 2018·Scientific Reports·Juraj BosákDavid Šmajs
Sep 12, 2018·Annual Review of Microbiology·Volkmar Braun
Jun 1, 1990·Molecular & General Genetics : MGG·R E Harkness, V Braun
Jul 1, 1981·Journal of Bacteriology·K SchallerV Braun
Apr 1, 1981·Journal of Bacteriology·K SchallerV Braun
Jun 1, 1983·Microbiological Reviews·B J Bachmann
Jan 1, 1992·Journal of Bacteriology·M Tuckman, M S Osburne

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