Penetration of Rickettsia tsutsugamushi into cultured mouse fibroblasts (L cells): an electron microscopic observation

Microbiology and Immunology
H UrakamiA Tamura

Abstract

The mechanism of penetration of purified Rickettsia tsutsugamushi (Gilliam strain) into cultured mouse fibroblasts (L cells) was examined by electron microscopy. After 10-40 min of infection, rickettsiae in the process of being phagocytized were often seen on the cell surface. These were restricted to the rickettsiae which seemed to be intact in morphology, while heavy plasmolyzed ones were never phagocytized. Additionally, rickettsiae were taken up individually into a phagosome, and phagocytosis of several rickettsiae together was rarely observed, except in the case of heat-inactivated microorganisms. In the cells, phagosomes whose membranes enclosed rickettsiae either tightly or loosely were seen. Rickettsiae in the loose phagosomes often showed signs of plasmolysis and were rarely released into the cell cytoplasm. Partial disintegration of phagosomal membranes and the escape of rickettsiae from the phagosomes were seen only in tight phagosomes. Large phagosomes containing a clump of several rickettsiae were observed occasionally, in which case the microorganisms were deformed and seemed to be denatured. From the above observations and the frequency of appearance of these different penetration stages in the specimens 10, 20, ...Continue Reading

References

Oct 1, 1978·Infection and Immunity·T S Walker, H H Winkler
Oct 1, 1976·Infection and Immunity·C L WissemanM R Jones
Jan 1, 1982·Microbiology and Immunology·A TamuraT Tsuruhara
Oct 1, 1982·Infection and Immunity·Y Rikihisa, S Ito
Mar 1, 1959·The Journal of Experimental Medicine·Z A COHNT K SAWYER

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Citations

Mar 6, 2004·Microbial Pathogenesis·Hang-Rae KimIk-Sang Kim
Feb 28, 2001·Microbes and Infection·S Y SeongI S Kim
Jan 17, 2008·Nature Reviews. Microbiology·Kevin Hybiske, Richard S Stephens
Jun 1, 1992·APMIS : Acta Pathologica, Microbiologica, Et Immunologica Scandinavica·K IwamasaY Kobayashi
Apr 16, 2004·Clinical Microbiology Reviews·Douglas A DrevetsRonald A Greenfield
Jun 15, 2013·Emerging Infectious Diseases·Meng ZhangHui-Li Yang
May 7, 2014·Proceedings of the National Academy of Sciences of the United States of America·Kenji WatanabeHiroaki Noda
Jan 1, 1985·Microbiology and Immunology·K TakahashiA Tamura
Aug 21, 2015·PLoS Pathogens·Kristen E Rennoll-BankertAbdu F Azad
Jan 1, 1990·Annals of the New York Academy of Sciences·H Li, D H Walker
Feb 2, 2018·Clinical Microbiology Reviews·Fabián E DíazAlexis M Kalergis
May 1, 1991·European Journal of Epidemiology·A TamuraN Ohashi
Feb 19, 2020·Pathogens and Disease·Joseph D ThiriotAlfredo G Torres
Oct 3, 2018·Tropical Medicine and Infectious Disease·Alison Luce-FedrowAllen L Richards
Jan 8, 2020·BMC Microbiology·Chien-Chung ChaoWei-Mei Ching
Jun 24, 2016·Microbiology Spectrum·Kevin Hybiske, Richard Stephens
Aug 1, 1989·Journal of Clinical Microbiology·H UrakamiA Tamura

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