PMID: 807157May 1, 1975

Penicillin-resistant mechanisms in Pseudomonas aeruginosa: binding of penicillin to Pseudomonas aeruginosa KM 338

Antimicrobial Agents and Chemotherapy
H SuginakaS Kotani

Abstract

A comparison of the binding of radioactive penicillin G to whole cells and the membrane fraction derived from Pseudomonas aeruginosa KM 338 was made. This organism has intrinsic resistance to penicillin. The binding to the membrane fraction which catalyzed peptidoglycan synthesis followed saturation type kinetics and saturation was achieved at approximately 2 nmol of penicillin G per ml, whereas binding to the whole cells was entirely of the nonsaturation type. The binding of carbenicillin to the membrane fraction was determined by competition between radioactive penicillin G and unlabeled carbenicillin for the binding sites. It was bound at the same sites in almost the same manner. When whole cells were pretreated with high concentration of unlabeled penicillin G or carbenicillin, the subsequent binding of radioactive penicillin G to the membrane fraction from carbenicillin-treated cells was entirely nonspecific, but with penicillin G-pretreated cells it was still specific. There was apparently specific binding of radioactive penicillin G to ethylenediaminetetraacetate-treated cells. P. aeruginosa KM 338 had an extremely low activity of beta-lactamase compared with other enzyme-producing organisms. This enzyme from P. aerugino...Continue Reading

References

Dec 31, 1971·Proceedings of the Royal Society of London. Series B, Containing Papers of a Biological Character·J L StromingerG G Wickus
Jan 1, 1972·Archiv für Mikrobiologie·H H MartinH J Preusser
Dec 18, 1972·European Journal of Biochemistry·H D Heilmann
Sep 22, 1965·Biochemical and Biophysical Research Communications·J M Hamilton-Miller
Feb 1, 1969·Canadian Journal of Microbiology·R G EAGON
Jun 13, 1970·Nature·P D FullbrookB Slocombe
Aug 1, 1952·Journal of General Microbiology·J H HUMPHREY, J W LIGHTBOWN
Oct 1, 1956·Biochimica Et Biophysica Acta·R REPASKE
May 18, 1964·Biochimica Et Biophysica Acta·J D DUERKSEN

Citations

Nov 1, 1983·Infection·B Wiedemann, A Seeberg
Jun 10, 1976·The New England Journal of Medicine·A L Smith
Sep 1, 1980·Antimicrobial Agents and Chemotherapy·P B Percheson, L E Bryan
Feb 1, 1982·Antimicrobial Agents and Chemotherapy·A J Godfrey, L E Bryan
Oct 1, 1984·Zentralblatt Für Bakteriologie, Mikrobiologie, Und Hygiene. Series A, Medical Microbiology, Infectious Diseases, Virology, Parasitology·E I el-Nima
Jun 9, 2009·Burns : Journal of the International Society for Burn Injuries·Farhat UllahJawad Ahmed

Related Concepts

Van-Pen-G
Penicillin Resistance
Penicillin
Pseudomonas aeruginosa

Related Feeds

CRISPR Screens in Drug Resistance

CRISPR-Cas system enables the editing of genes to create or correct mutations. This feed focuses on the application of CRISPR-Cas system in high-throughput genome-wide screens to identify genes that may confer drug resistance.

Bacterial Cell Wall Structure

Bacterial cell walls are made of peptidoglycan (also called murein), which is made from polysaccharide chains cross-linked by unusual peptides containing D-amino acids. Here is the latest research on bacterial cell wall structures.

Bacterial Cell Wall Structure (ASM)

Bacterial cell walls are made of peptidoglycan (also called murein), which is made from polysaccharide chains cross-linked by unusual peptides containing D-amino acids. Here is the latest research on bacterial cell wall structures.

Related Papers

Clinical Infectious Diseases : an Official Publication of the Infectious Diseases Society of America
R E Hancock
British Medical Journal
C F DrysdaleJ Brodie
Proceedings of the Royal Society of London. Series B, Containing Papers of a Biological Character
J L StromingerG G Wickus
© 2021 Meta ULC. All rights reserved