Pentobarbital affects transepithelial electrophysiological parameters regulated by protein kinase C in rat distal colon

Digestive Diseases and Sciences
R M SimonsJ M Mullin

Abstract

For rat distal colon, the transepithelial electrical parameters, short circuit current (Iscc) and transepithelial electrical resistance (TER), respectively, measure net transepithelial electrolyte transport activity and the barrier function of the epithelium. Studies with a variety of epithelial cell cultures have shown greater than 90% decreases of TER within minutes of exposure of in vitro cell sheets to phorbol esters. The phorbol ester and protein kinase C (PKC) activator, phorbol dibutyrate (PDBU), was observed to produce an over 100% elevation of Iscc but only a small yet significant 20-30% decrease of TER across rat distal colon. Inhibition of the above effects of PDBU by the PKC inhibitor bisindolylmaleimide (GFX) is further evidence that in rat distal colon, Iscc and TER are under regulatory control by PKC. When animals received anesthesia with intraperitoneal pentobarbital prior to removal of the colon, the effect of PDBU on Iscc was significantly reduced, and the effect of PDBU on TER was almost completely inhibited. This effect of pentobarbital on PKC-mediated transepithelial permeability parameters is consistent with the known ability of anesthetics to alter protein kinase C activity. Exposure of rat colon to pento...Continue Reading

Citations

May 9, 2000·American Journal of Physiology. Gastrointestinal and Liver Physiology·S UmarA P Morris
Feb 24, 2001·Annals of the New York Academy of Sciences·J GrootM Perdue
Aug 27, 2010·American Journal of Physiology. Cell Physiology·Arivudainambi RamalingamJames M Mullin

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.