Pentose Metabolism in Saccharomyces cerevisiae: The Need to Engineer Global Regulatory Systems

Biotechnology Journal
Venkatesh Endalur Gopinarayanan, Nikhil U Nair

Abstract

Extending the host substrate range of industrially relevant microbes, such as Saccharomyces cerevisiae, has been a highly-active area of research since the conception of metabolic engineering. Yet, rational strategies that enable non-native substrate utilization in this yeast without the need for combinatorial and/or evolutionary techniques are underdeveloped. Herein, this review focuses on pentose metabolism in S. cerevisiae as a case study to highlight the challenges in this field. In the last three decades, work has focused on expressing exogenous pentose metabolizing enzymes as well as endogenous enzymes for effective pentose assimilation, growth, and biofuel production. The engineering strategies that are employed for pentose assimilation in this yeast are reviewed, and compared with metabolism and regulation of native sugar, galactose. In the case of galactose metabolism, multiple signals regulate and aid growth in the presence of the sugar. However, for pentoses that are non-native, it is unclear if similar growth and regulatory signals are activated. Such a comparative analysis aids in identifying missing links in xylose and arabinose utilization. While research on pentose metabolism have mostly concentrated on pathway ...Continue Reading

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Citations

Jun 30, 2019·Yeast·Margareth Andrea PatiñoBoris U Stambuk
Dec 18, 2019·Biochemical Society Transactions·Marcos Valenzuela-Ortega, Christopher E French
Sep 6, 2019·Brazilian Journal of Microbiology : [publication of the Brazilian Society for Microbiology]·Ronivaldo Rodrigues da SilvaEleni Gomes
Aug 20, 2020·Applied Microbiology and Biotechnology·Josman VelascoFernando Segato
Nov 28, 2020·Current Opinion in Biotechnology·Jae Won LeeYong-Su Jin

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