Pentylenetetrazol-induced status epilepticus up-regulates the expression of glucose transporter mRNAs but not proteins in the immature rat brain

Brain Research
Astrid NehligS J Vannucci

Abstract

Prolonged pentylenetetrazol (PTZ)-induced seizures increase cerebral energy demands in a region-specific manner. During PTZ seizures, cerebral glucose utilization increases over control levels in all brain regions at 10 days while 21-day-old rats exhibit increases, decreases or no change. To explore the effects of such acute changes in metabolic demand on the expression of glucose transporter proteins mediating glucose delivery to brain, we studied the consequences of PTZ seizures on GLUT1 and GLUT3 mRNAs and proteins between 1 and 72 h after seizure induction. At both ages, seizures induced a rapid up-regulation of GLUT1 and GLUT3 mRNAs which was prominent at 1 and 4 h, and was greater at 10 than at 21 days. By 24 h and 72 h, the levels of the mRNAs of the two transporter returned to control levels or were slightly down-regulated. The levels of GLUT1 and GLUT3 proteins were not affected by the seizures and only scattered decreases in GLUT3 protein were recorded, mainly in midbrain-brainstem areas. These data show that acute pentylenetetrazol seizures induce a rapid up-regulation of the GLUT1 and GLUT3 mRNAs, but do not result in measurable increases in protein levels, suggesting translational regulation.

References

May 1, 1977·Journal of Neurochemistry·A G ChapmanB K Siesjö
Jan 31, 1992·Biochemical and Biophysical Research Communications·F MaherI A Simpson
Apr 1, 1990·Brain Research. Developmental Brain Research·D M FerrieroF R Sharp
Apr 1, 1989·Journal of Neuroscience Research·D Z GerhartL R Drewes
Jun 1, 1989·The American Journal of Physiology·D G FujikawaC G Wasterlain
Jun 28, 1988·Brain Research·D G FujikawaC G Wasterlain
Nov 1, 1987·Annals of Neurology·J W MillerJ A Ferrendelli
Oct 1, 1983·Physiological Reviews·W M Pardridge
Aug 1, 1995·Proceedings of the National Academy of Sciences of the United States of America·M S LawrenceR M Sapolsky
Oct 1, 1994·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·F MaherI A Simpson
Jan 1, 1994·Journal of Neurochemistry·S J Vannucci
Apr 15, 1993·Proceedings of the National Academy of Sciences of the United States of America·D Y HoR M Sapolsky
Feb 1, 1993·Progress in Neurobiology·A Nehlig, A Pereira de Vasconcelos
Mar 1, 1993·Journal of Cerebral Blood Flow and Metabolism : Official Journal of the International Society of Cerebral Blood Flow and Metabolism·F MaherI A Simpson
Jan 1, 1996·Journal of Cerebral Blood Flow and Metabolism : Official Journal of the International Society of Cerebral Blood Flow and Metabolism·S J VannucciR C Vannucci
Jul 1, 1996·Journal of Neuropathology and Experimental Neurology·K M GronlundL R Drewes
Oct 21, 1998·Developmental Neuroscience·S J VannucciI A Simpson
Feb 12, 1999·Brain Research. Developmental Brain Research·N PineauA Nehlig
Feb 23, 1999·Journal of Cerebral Blood Flow and Metabolism : Official Journal of the International Society of Cerebral Blood Flow and Metabolism·M J FernandesA Nehlig
Oct 10, 2003·American Journal of Physiology. Endocrinology and Metabolism·Susan J Vannucci, Ian A Simpson
Aug 1, 1992·Molecular and Cellular Neurosciences·C A BondyJ Zhou

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Citations

Jun 26, 2008·American Journal of Physiology. Endocrinology and Metabolism·Ian A SimpsonSusan J Vannucci
May 23, 2012·ASN Neuro·Gerald A Dienel
Apr 13, 2007·International Journal of Developmental Neuroscience : the Official Journal of the International Society for Developmental Neuroscience·Qian JiangYuwu Jiang
Aug 25, 2012·Evidence-based Complementary and Alternative Medicine : ECAM·Ming Wai HungSimon Ming Yuen Lee
Feb 23, 2017·Journal of Child Psychology and Psychiatry, and Allied Disciplines·Sören MerkerKlaus-Peter Lesch
Aug 29, 2012·Developmental Medicine and Child Neurology·Svetlana GataullinaPascale De Lonlay
Sep 27, 2019·Neurocritical Care·Helene HurthFlorian Heinrich Ebner
Oct 31, 2020·Molecular Neurobiology·Igor Santana de MeloOlagide Wagner de Castro

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