PEOPLE: PatiEnt prOstate samPLes for rEsearch, a tissue collection pathway utilizing magnetic resonance imaging data to target tumor and benign tissue in fresh radical prostatectomy specimens

The Prostate
Susan HeaveyHayley C Whitaker

Abstract

Over 1 million men are diagnosed with prostate cancer each year worldwide, with a wide range of research programs requiring access to patient tissue samples for development of improved diagnoses and treatments. A random sampling of prostate tissue is sufficient for certain research studies; however, there is growing research need to target areas of the aggressive tumor as fresh tissue. Here we set out to develop a new pathway "PEOPLE: PatiEnt prOstate samPLes for rEsearch" to collect high-quality fresh tissue for research use, using magnetic resonance imaging (MRI) to target areas of tumor and benign tissue. Prostate tissue was sampled following robotic radical prostatectomy, using MRI data to target areas of benign and tumor tissue. Initially, 25 cases were sampled using MRI information from clinical notes. A further 59 cases were sampled using an optimized method that included specific MRI measurements of tumor location along with additional exclusion criteria. All cases were reviewed in batches with detailed clinical and histopathological data recorded. For one subset of samples, DNA was extracted and underwent quality control. Ex vivo culture was carried out using the gelatin sponge method for an additional subset. Tumor wa...Continue Reading

References

Nov 1, 1994·The Prostate·T M Wheeler, R M Lebovitz
May 30, 2008·International Journal of Surgical Pathology·Prasanna SooriakumaranStephen Em Langley
May 5, 2012·Diagnostic Molecular Pathology : the American Journal of Surgical Pathology, Part B·Raquel EsguevaUNKNOWN Weill Cornell Medical College
Jun 12, 2013·Nature Reviews. Urology·Margaret M CenteneraLisa M Butler
Mar 30, 2017·British Journal of Cancer·Lucy A M SimmonsHashim U Ahmed
Nov 9, 2017·Journal of Translational Medicine·Giacomo MiserocchiToni Ibrahim
Oct 20, 2018·Frontiers in Oncology·Arjanneke F van de MerbelGabri van der Pluijm

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Methods Mentioned

BETA
biopsies
biopsy
imaging techniques
Assay

Software Mentioned

IMPAX
PEOPLE

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