PMID: 9168472May 1, 1997Paper

Pep7p provides a novel protein that functions in vesicle-mediated transport between the yeast Golgi and endosome

Molecular Biology of the Cell
G C WebbE W Jones

Abstract

Saccharomyces cerevisiae pep7 mutants are defective in transport of soluble vacuolar hydrolases to the lysosome-like vacuole. PEP7 is a nonessential gene that encodes a hydrophilic protein of 515 amino acids. A cysteine-rich tripartite motif in the N-terminal half of the polypeptide shows striking similarity to sequences found in many other eukaryotic proteins. Several of these proteins are thought to function in the vacuolar/lysosomal pathway. Mutations that change highly conserved cysteine residues in this motif lead to a loss of Pep7p function. Kinetic studies demonstrate that Pep7p function is required for the transport of the Golgi-precursors of the soluble hydrolases carboxypeptidase Y, proteinase A, and proteinase B to the endosome. Integral membrane hydrolase alkaline phosphatase is transported to the vacuole by a parallel intracellular pathway that does not require Pep7p function. pep7 mutants accumulate a 40-60-nm vesicle population, suggesting that Pep7p functions in a vesicle consumption step in vesicle-mediated transport of soluble hydrolases to the endosome. Whereas pep7 mutants demonstrate no defects in endocytic uptake at the plasma membrane, the mutants demonstrate defects in transport of receptor-mediated macr...Continue Reading

References

Jan 1, 1992·Annual Review of Biochemistry·N K PryerR Schekman
Oct 25, 1991·Nucleic Acids Research·J HillG Donald
Jan 1, 1989·Annual Review of Cell Biology·S Kornfeld, I Mellman
Feb 1, 1989·The Journal of Cell Biology·C M MoehleE W Jones
Jun 1, 1989·Trends in Genetics : TIG·T J WhiteH A Erlich
Sep 1, 1989·Proceedings of the National Academy of Sciences of the United States of America·R A PrestonE W Jones
Dec 1, 1986·Proceedings of the National Academy of Sciences of the United States of America·V A BankaitisS D Emr
Aug 1, 1981·Cell·P NovickR Schekman
Oct 1, 1995·The Journal of Cell Biology·E Harsay, A Bretscher
Nov 3, 1994·Nature·J E Rothman
Jul 21, 1994·Nature·S Ferro-Novick, R Jahn
Jul 1, 1993·The Journal of Cell Biology·N G DavisG F Sprague
Mar 15, 1996·Science·R Schekman, L Orci
Jan 1, 1996·Yeast·H B Van Den HazelJ R Winther

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Citations

Jun 8, 1999·Chemistry and Physics of Lipids·J M GaullierH Stenmark
Jun 30, 2000·Current Opinion in Cell Biology·S K Lemmon, L M Traub
Jun 30, 2000·Current Opinion in Cell Biology·N L Catlett, L S Weisman
Apr 30, 2008·Proceedings of the National Academy of Sciences of the United States of America·Rekha PuriaMaria E Cardenas
Feb 26, 2000·Molecular Biology of the Cell·S R GerrardT H Stevens
Mar 30, 2004·Molecular Biology of the Cell·Shoba SubramanianElizabeth W Jones
Apr 20, 2005·Genome Research·Becky IrwinSuzanne Sandmeyer
Jan 19, 2006·Proceedings of the National Academy of Sciences of the United States of America·Shoba SubramanianElizabeth W Jones
Mar 27, 2007·Biochimica Et Biophysica Acta·Thomas Strahl, Jeremy Thorner
Aug 17, 1999·Current Opinion in Cell Biology·M G Waters, S R Pfeffer
May 26, 2005·Biochimica Et Biophysica Acta·Katherine Bowers, Tom H Stevens
Aug 26, 1998·Biochimica Et Biophysica Acta·E Conibear, T H Stevens
Apr 12, 2016·Biology of the Cell·Kyoungtae Kim, Shiva Kumar Goud Gadila
Nov 18, 2003·Annual Review of Genetics·Lois S Weisman
Oct 3, 1999·The Journal of Biological Chemistry·A SimonsenH Stenmark
Sep 27, 2006·The Journal of Biological Chemistry·Jelena VukmiricaMireille Cormont
Oct 2, 2008·Anti-cancer Drugs·Chuang-Xin LuYan Lei
Jul 29, 2020·Frontiers in Cell and Developmental Biology·Logan R Hurst, Rutilio A Fratti
Aug 18, 2001·The Journal of Biological Chemistry·M MariM Cormont
Aug 30, 2001·The Journal of Biological Chemistry·M E ShinC G Burd
Feb 24, 2001·Traffic·M G Waters, F M Hughson
Dec 31, 2019·Fungal Genetics and Biology : FG & B·Tim M van LeeuweArthur F J Ram

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